Aim of the Research. The aim of the research is to examine if there is a difference in opinions and knowledge about posthumous organ donation between students from healthcare and non-healthcare fields. Study Design. A cross-sectional study. Participants and Methods. The study included 103 participants, with 43 students from the Electrical Engineering and Computing study and 60 students from the Medical study. The research was conducted using an anonymous survey questionnaire composed of three parts: the first part collects demographic data, the second part examines opinions and willingness to donate organs posthumously, and the third part assesses the level of knowledge about transplantation. Results. A large number of respondents consider transplantation a positive form of treatment. Twice as many medical students would donate their organs to anyone in need. The main reason for unwillingness, according to the respondents, is a lack of information. Medical students significantly more often cite professional literature as their source of knowledge, while non-healthcare students cite media and the internet. The organ they are most unwilling to donate is the eyes. Conclusion. Medical students showed greater willingness to donate organs and greater knowledge about the topic, leading to the conclusion that education increases awareness and willingness to donate organs posthumously.