prikaz prve stranice dokumenta Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode
  Download
PDF 2.46 MB
master's thesis
Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. urn:nbn:hr:152:741997
Marković, Marina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

Cite this document

Marković, M. (2024). Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:741997

Marković, Marina. "Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:741997

Marković, Marina. "Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2024. https://urn.nsk.hr/urn:nbn:hr:152:741997

Marković, M. (2024). 'Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:152:741997

Marković M. Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2024 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:152:741997

M. Marković, "Analiza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2024. Available at: https://urn.nsk.hr/urn:nbn:hr:152:741997

Metadata
TitleAnaliza raznolikosti delta lanca T staničnih receptora perifernih gamadelta T stanica oboljelih od vulgarne psorijaze primjenom TCRSeq metode
Title (english)Analysis of T cell receptor delta chain diversity in gamma-delta T cells of psoriasis vulgaris patients using TCRSeq methodology
AuthorMarina Marković
MentorStana Tokić (mentor)
Committee memberMario Štefanić (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberStana Tokić (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2024-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Ciljevi istraživanja: Istraživanjem se istražio i usporedio repertoar delta TCR lanaca CD3+γδTCR+ γδ T-limfocita u koži zdravih pojedinaca i osoba oboljelih od vulgarne psorijaze te ustanovila povezanost otkrivenih delta TCR klonotipova s obilježjima i težinom ove bolesti. Nacrt studije: Studija je ustrojena kao istraživanje parova. Ispitanici i metode: Iz tkivnih leukocita dobivenih uzorkovanjem kože zdravih ispitanika i bolesnika s vulgarnom psorijazom izolirana je RNK te je njenim sekvenciranjem analizirana raznolikost delta lanaca T-staničnog receptora gama delta T-limfocita. Rezultati: Pronađeno je 166 jedinstvenih klonotipova, od kojih 164 privatne i 2 dijeljene varijante. Klonotipovi srednje do velike učestalosti bili su najzastupljeniji s prosječnom dužinom CDR3 regije 14 – 17 aminokiselina. Najučestalije genske varijante TRDV2, TRDV1 i TRDV3 najčešće su udruživane s genima TRDJ1, TRDJ3 i TRDJ4. Dužina trajanja psorijaze rasla je s porastom izražaja gena TRDV2, a veličina PASI i DLQI indeksa negativno je korelirala s izražajem gena TRDV1, TRDV3 i TRDJ4. Shannon-Wiener i iSimpson indeks negativno su korelirali s PASI. Zaključak: TRD repertoar kožnih leukocita čini mali broj jedinstvenih klonotipova, izrazito privatnog i individualnog karaktera srednje do velike učestalosti. Indeksi raznolikosti repertoara i izražaj gena TRDV1, TRDV3, TRDJ4 negativno koreliraju s kraćim i blažim oblikom bolesti, dok TRDV2 pozitivno korelira s dužim trajanjem psorijaze.
Abstract (english)
Objectives: The study investigated and compared the repertoire of delta TCR chains of CD3+γδTCR+ γδ T-lymphocytes in the skin of healthy individuals and people suffering from psoriasis vulgaris and established the association of detected delta TCR clonotypes with the characteristics and severity of this disease. Study design: The study was conducted as a case-control study. Participants and methods: RNA was isolated from tissue leukocytes obtained by sampling the skin of healthy subjects and patients with psoriasis vulgaris, and the delta chain diversity of the gamma delta T-lymphocyte T-cell receptor was analyzed by sequencing. Results: 166 unique clonotypes were found, of which 164 were private and 2 shared variants. Medium to high frequency clonotypes were the most abundant, with an average length of the CDR3 region of 14 – 17 amino acids. The most frequent gene variants TRDV2, TRDV1 and TRDV3 were most often combined with the genes TRDJ1, TRDV3 and TRDJ4. The duration of psoriasis increased with the higher expression of TRDV2, and the size of PASI and DLQI indices negatively correlated with the expression of TRDV1, TRDJ3 and TRDJ4. ShannonWiener and iSimpson indices negatively correlated with PASI. Conclusion: The TRD repertoire of skin leukocytes consists of a small number of unique clonotypes, with an extremely private and individual character, in medium to high frequency. The repertoire diversity indices and the expression of the genes TRDV1, TRDV3 and TRDJ4 negatively correlate with a shorter and milder form of the disease, while TRDV2 positively correlates with a longer duration of psoriasis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:741997
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-10-29 12:39:08