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master's thesis
Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2017. urn:nbn:hr:152:053985
Jakuš, Nikolina
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Jakuš, N. (2017). Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:053985

Jakuš, Nikolina. "Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:053985

Jakuš, Nikolina. "Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:053985

Jakuš, N. (2017). 'Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 28 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:053985

Jakuš N. Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2024 November 28] Available at: https://urn.nsk.hr/urn:nbn:hr:152:053985

N. Jakuš, "Antiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:053985

Metadata
TitleAntiproliferativni učinak novih N-9 sulfonilurea na stanicama koje rastu u 2D i 3D kulturi
Title (english)Antiproliferative effects of novel N-9 sulphonylurea derivatives on 2D and 3D cell cultures
AuthorNikolina Jakuš
MentorLjubica Glavaš-Obrovac (mentor)
Committee memberSaška Marczi (predsjednik povjerenstva)
Committee memberTatjana Belovari (član povjerenstva)
Committee memberLjubica Glavaš-Obrovac (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2017-05-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Abstract
Uvod: Vodeći uzrok smrtnosti u svijetu čine tumorske bolesti koje nastaju kao posljedica zagađenja okoliša i starenja svjetske populacije. Velika je potreba za pronalaskom učinkovitih antitumorskih lijekova. Modificirani nukleozidi i baze nukleinskih kiselina predmet su mnogih istraživanja radi njihove potencijalne antitumorske i antivirusne aktivnosti. Cilj istraživanja: Odrediti razlikuje li se antiproliferativni potencijal derivata N-9 sulfonil urea na rast normalnih i tumorskih stanica u dvodimenzionalnim i trodimenzionalnim kulturama in vitro. Materijali i metode: Učinci derivata N-9 sulfonilureje na rast stanica određeni su MTT testom na panelu humanih tumorskih stanica u 2D kulturi (HeLa, CaCo-2, NCI-H358, K562, Raji, HuT 78, MOLT4 i CCRF-CEM), normalnim stanicama (MDCK1) i 3D kulturi stanica HeLa, CaCo-2 i NCI-H358. Promjene u staničnom ciklusu određene su protočnom citometrijom. Rezultati: Testirani derivati N-9 sulfonilureja prikazuju antiproliferativni učinak ovisno o pri primijenjenoj koncentraciji i staničnoj liniji u 2D i 3D kulturi. Najbolji inhibicijski učinak na rast tumorskih stanica imao je spoj R-194. Raji stanice nakon izlaganja spoju R-194 pokazuju statistički značajne promjene u udjelu stanica pojedinih faza staničnog ciklusa, a posebno nakupljanje stanica u sub G0 fazi u odnosu na kontrolne stanice. Zaključak: Razlikuje se odgovor stanica koje rastu u 2D i 3D kulturi na toksične učinke novositetiziranih derivata N-9-sulfonilureje. Najučinkovitiji je derivat R-194, koji značajno mijenja udio Raji stanica u pojedinim fazama staničnog ciklusa u odnosu na normalne stanice.
Abstract (english)
Introduction: Leading cause of death in the world are tumour diseases as a result of environmental pollution and aging of the world population. Therefore, there is a huge need for discovering effective anticancer drugs. Nucleoside analogs are the subject of numerous researches because of their potential anti-tumour and antiviral activity. Study aim: The aim was to determine whether there is difference in anti-proliferation potential of N-9 sulfonylurea growth of normal and tumour cells in the two-dimensional and three-dimensional cultures in vitro. Material and methods: Effects of N-9 sulfonylureas are set on the panel of human tumour cells in 2D culture (HeLa, CaCo-2, NCI-H358, K562, Raji, HuT 78, MOLT4 and CCRF-CEM), normal cells (MDCK1) and 3D culture (HeLa, CaCo-2, NCI-H358). Changes in cell cycle were determined by flow cytometric analysis. Results: Test of N-9 sulfonylureas shows antiproliferative activity depending on concentration and prepared in a cell line of 2D and 3D culture. The best inhibitory effect on tumour cell growth is R-194 compound. Raji human cell line, after the exposure to the R-194 compound, indicated a statistically significant change in the proportion of cells in cell cycle, and particular accumulation of cells in sub G0 phase compared to control cells. Conclusion: There were different responses of cells grown in 2D and 3D culture on toxic effects of N-9-sulphonylurea. The most effective derivative is R-194, which significantly changes the proportion of Raji cells in specific phases of the cell cycle compared to control cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:053985
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-12 13:22:32