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master's thesis
Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2017. urn:nbn:hr:152:601475
Meščić, Gordana
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Meščić, G. (2017). Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora (Master's thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:601475

Meščić, Gordana. "Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:601475

Meščić, Gordana. "Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora." Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:601475

Meščić, G. (2017). 'Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora', Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 05 March 2025, https://urn.nsk.hr/urn:nbn:hr:152:601475

Meščić G. Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora [Master's thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2025 March 05] Available at: https://urn.nsk.hr/urn:nbn:hr:152:601475

G. Meščić, "Utjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora", Master's thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:601475

Metadata
TitleUtjecaj kratkotrajnog unosa visokog prehrambenog unosa kuhinjske soli na dinamiku subpopulacija leukocita u krvi štakora
Title (english)The effect of short-term high salt diet on a dynamics of blood leukocyte's subpopulation in rats
AuthorGordana Meščić
MentorInes Drenjančević (mentor)
Committee memberAna Stupin (predsjednik povjerenstva)
Committee memberIvana Jukić (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2017-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
Cilj: Ocijeniti dinamiku aktivacije leukocita kod Sprague-Dawley štakora na LS i HS dijeti određivanjem izražajnosti komplementarnih liganada za adhezijske molekule endotelnih stanica na različitim podskupinama leukocita. Metode: Zdravi SD štakori starosti 11 tjedana hranjeni su standardnom hranom 0,4% NaCl (NS grupa) ili hranom s 4% NaCl (VS grupa) 7 dana. Protočnom citometrijom iz uzoraka pune krvi određena je učestalost staničnih populacija te izražaj adhezijskih molekula. Rezultati:... More Učestalost staničnih populacija nije se razlikovala u eksperimentalnim skupinama. HS dijeta izazvala je značajno smanjenje leukocita s visokim izražajem CD49d +. Izražaj CD11b/c biljega na granulocitima nije promijenjen unosom soli. Mjerena je učestalost subpopulacija limfocita T između ispitivanih skupina i rezultati nisu pokazali statističku razliku. Medijan izražaja CD49d na T limfocitima nije promijenjen povećanim unosom prehrambene soli. Medijan izražaja CD11a biljega nije promijenjen u populaciji CD4 + limfocita, no statistički je vidljiv niži izražaj CD3 + CD4 + stanica s visokim izražajem CD11a biljega u HS skupini u usporedbi s LS skupinom. Zaključak: Visok unos soli dovodi do aktivacije imunološkog sustava, infiltracije stijenke krvnih žila leukocitima koja može voditi razvoju ateroskleoze. Smanjena regulacija izražaja CD49d biljega adhezijskih molekula na visokopozitivnim granulocitima CD49d te smanjenje izražaja CD3+ CD4+ stanica koje povećavaju izražaj CD11a molekule u HS skupini što sugerira da su navedene stanice migrirale kroz endotelni sloj krvnih žila do mjesta budućeg stvaranja plaka. Rezultati sugeriraju da povećan unos soli dovodi do interakcije specifične i urođene imunosti putem aktivacije monocita/makrofaga te CD3+CD4+ T limfocita. Less
Abstract (english)
Objective: To evaluate the dynamics of leukocyte activation in Sprague-Dawley rats on an LS and HS diet by determining the expression of complementary ligands for endothelial cell adhesion molecules on different leukocyte subgroups. Methods: Healthy SD rats aged 11 weeks were fed with standard food 0.4% NaCl (NS group) or food with 4% NaCl (VS group) for 7 days. Flow chart cytometry from full blood samples determines the frequency of cell populations and the expression of adhesion... More molecules. Results: The frequency of cell populations did not differ in experimental groups. HS dieting has caused a significant decrease in high expression CD49d + leukocytes. The expression of CD11b / c markers on granulocytes has not been changed by salt intake. The frequency of subpopulation of lymphocytes T between the examined groups was measured and the results showed no statistical difference. Median expression CD49d on T lymphocytes was not changed by increased intake of dietary salt. Median expression of CD11a markers was not changed in the CD4 + lymphocyte population but there was statistically lower CD3 + CD4 + expressing CD11a marking in HS group compared to LS group. Conclusion: High salt intake leads to activation of the immune system, leukocyte infiltration of the blood vessels that may lead to the development of atherosclerosis. Reduced regulation of expression of CD49d markers of adhesion molecules on high-release CD49d granulocytes and CD3 + CD4 + expression expressing CD11a molecule expression in HS group suggesting that these cells migrated through the endothelial layer of blood vessels to the site of future plaque formation. The results suggest that increased salt intake leads to the interaction of specific and innate immunity by activating monocytes / macrophages and CD3 + CD4 + T lymphocytes. Less
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:601475
Study programmeTitle: University graduate study
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra medicinsko laboratorijske dijagnostike (magistar/magistra medicinsko laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-13 09:34:39
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