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undergraduate thesis
Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom
Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine, 2017. urn:nbn:hr:152:732908
Tomac, Petra
Josip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek

Institutional repository: Repository of the Faculty of Medicine Osijek

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Tomac, P. (2017). Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom (Undergraduate thesis). Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek. Retrieved from https://urn.nsk.hr/urn:nbn:hr:152:732908

Tomac, Petra. "Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:732908

Tomac, Petra. "Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom." Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, 2017. https://urn.nsk.hr/urn:nbn:hr:152:732908

Tomac, P. (2017). 'Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom', Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:152:732908

Tomac P. Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom [Undergraduate thesis]. Osijek: Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:152:732908

P. Tomac, "Spolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom", Undergraduate thesis, Josip Juraj Strossmayer University of Osijek, Faculty of Medicine Osijek, Osijek, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:152:732908

Metadata
TitleSpolno uvjetovane razlike u izražaju antioksidativnih gena u aorti Sprague-Dawley štakora hranjenih visoko-masnom- visoko-slatkom hranom
Title (english)Sex-dependant differences in antioxidative genes expression in aorta of Sprague-Dawley rats fed with high-fat-high-sugar diet
AuthorPetra Tomac
MentorInes Drenjančević (mentor)
Committee memberIvana Jukić (predsjednik povjerenstva)
Committee memberAna Stupin (član povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
GranterJosip Juraj Strossmayer University of Osijek
Faculty of Medicine Osijek
Osijek
Defense date and country2017-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Abstract
CILJ: Cilj istraživanja bio je utvrditi spolno uvjetovane razlike u izražaju gena antioksidacijskih enzima u tkivu aorti preddijabetičkih Sprague-Dawley štakora koji su konzumirali hranu bogatu mastima i šećerima. MATERIJALI I METODE: Zdravi Sprague-Dawley štakori, oba spola, starosti 10 mjeseci podijeljeni su u četiri skupine: kontrolna skupina, HSHFD skupina, HSHFD + metformin skupina i HSHFD + liraglutid skupina te su uvedeni dijetni protokoli u trajanju od 4 mjeseca. Iz tkiva aorte izmjeren je izražaj gena antioksidacijskih enzima (Cu/Zn SOD, MnSOD, EC-SOD, GPx1, 4 i katalaze) pcr metodom u stvarnom vremenu. REZULTATI: Terapija liraglutidom izazvala je gubitak tjelesne mase kod obaju spolova, ali značajno kod ženskih štakorica. Izražaj EC-SOD, GPx1 i katalaze povećan je u aortama štakorica skupine HSHFD + liraglutid u usporedbi s ostalim ispitivanim skupinama. U HSHFD + liraglutid skupini štakorica izražaj gena CuZnSOD značajno je povećan u usporedbi s HSHFD i HSHFD + metformin skupinom. Izražaj GPx4 smanjen je u skupini HSHFD + metformina u usporedbi s ostalim skupinama ženskih aorti. Izražaj MnSOD-a nije se značajno promijenio među skupinama ženskog spola. U muškoj skupini štakora izražaj se antioksidacijskih enzima nakon HSHFD prehrane nije značajno promijenio. ZAKLJUČAK: Hrana s visokim udjelom masnoće dovodi do pojave pretilosti kod obaju spolova. Promjene antioksidacijskih enzima značajnije su kod ženskog spola. Terapija je liraglutidom značajno povećala antioksidacijski status kod ženskog spola. Također, metformin nije značajno utjecao na promjene antioksidacijskogs statusa u ispitivanim skupinama.
Abstract (english)
AIM: The aim of the study was to determine the sex differences in the expression of antioxidative enzymes in aortas of prediabetic Sprague-Dawley rats which consumed fat and sugar rich food. MATERIALS AND METHODS: Healthy Sprague-Dawley rats, both sexes, aged 10 months were divided into four groups: control group, HSHFD group, HSHFD + metformin group and HSHFD + liraglutide group. They were included in 4 month diet protocols. From aortic tissue was measured gene expression of antioxidative enzymes (Cu/Zn SOD, MnSOD, EC-SOD, GPxl, 4 and catalase) by PCR in real time. RESULTS: Liraglutide therapy caused loss of body weight in both sexes, but significant in female rats. The expression of EC-SOD, GPx1 and catalase was increased in aorta of HSHFD + liraglutide rats compared to other investigated groups. In the HSHFD + liraglutide group, Cu/ZnSOD was significantly increased comapred to HSHFD and HSHFD + metformin groups. Expression of GPx4 was decreased in the HSHFD + metformin group compared to other female groups. Expression of MnSOD did not change significantly between groups. In male group of rats expression of antioxidative enzymes after HSHFD diet was decreases, and was not affected by antidiabetic treatment. CONCLUSION: Foods with high fat content leads to obesity in both sexes and increased oxidative stress. Expression of antioxidative enzymes are more significant in female experimental group. Liraglutide therapy has significantly increased antioxidative status in femals. Also, metformin did not significantly affect on the reduction of antioxidative stress caused by obesity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:152:732908
Study programmeTitle: University undergraduate study
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-09-14 12:00:24