Objective: The aim of this research is to assess the awareness of the importance of voluntary blood donation among students of the third year of the University Undergraduate Study of Nursing and the students of the third year of the University Integrated Undergraduate and Graduate Study of Medicine. It is also a matter of determining whether there is a difference in age, gender, prior education, and direction of study. Organisation of the study: The study is structured as a cross-sectional study Subjects and methods: The respondents were third year students of the university undergraduate study of nursing at the Faculty of Medicine Osijek, where out of the 64 enrolled students 53 of them participated in the study, and third year students of Integrated Undergraduate and Graduate Study of Medicine, where out of 78enrolled students 62 of them participated in the research. The research was conducted at the Faculty of Medicine Osijek in 2017. For the purpose of this research, a self-constructed questionnaire was used, consisting of 19 closed-type questions to which a person should answer by choosing one of the offeredanswers, among which is one open question, to which a person should answer by entering age-related data. The questionnaire consists of general demographic data (age, gender, direction, years of study, pre-education) and questions for evaluation of the ways of giving blood. Results: Blood is donated by 28 (24%) respondents. Eighteen (66%) of them are undergraduate university students of nursing. The reason for giving blood to 22respondentsis the wish to help others. Respondentswho graduated frommedical or vocational school donate blood more frequentlycompared to those who graduated from secondary school. Conclusion: Most students are aware of the facts of the necessity to donate blood, and are planning to donate it or think they will do so in the next six months. Blood is more donated by students of University Undergraduate Study of Nursing.