Objectives: To examine the knowledge and personal behaviours of nurses about the prevention of cervical cancer and to establish whether there is a connection between the knowledge and personal behaviour of the nurses at the Osijek Health Centre. Study Design: The study is cross-sectional. Participants and Methods: The participants are nurses employed in the practices and on the premises of the Osijek Health Centre. The survey questionnaires were completed by 114 nurses. Results: The average age of the participants is 44 years. 14% of them have a person in their family suffering from cervical cancer. Most participants are familiar with the risk factors, causes, symptoms, and ways of preventing cancer. 43.8% of the participants possess good knowledge about cancer. Over the last 12-month period 53.5% of the participants had a gynaecological examination and 13.2% of them 3 and more years ago. The reasons for visiting gynaecologist more often for those with a positive family anamnesis are health problems or other reasons. The participants with a negative anamnesis often had preventive examinations done. 49.1% of the participants had their last Pap test done 12 months ago, 14% three and more years ago. Out of 93.9% of the participants aged 25 to 64, 36.4% received a call from the Ministry of Health. 73.7% responded to the call. 55 examinees (48.7%) have a female person aged 18 to 35 in their family. 30 (53.6%) had an average impact on their cancer prevention activities. Conclusion: Slightly less than half of the participants possess good knowledge about cervical cancer. Most participants had the last Pap test done in the last 12 months. There are no significant differences in the behaviour regarding the knowledge about cervical cancer.