prikaz prve stranice dokumenta Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa
  Download
PDF 1.36 MB
master's thesis
Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa
Split: University of Split, School of Medicine, 2020. urn:nbn:hr:171:853736
Ćavar, Ivna
University of Split
School of Medicine
Department of Anatomy, Histology and Embryology

Institutional repository: MEFST Repository

Cite this document

Ćavar, I. (2020). Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:853736

Ćavar, Ivna. "Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:853736

Ćavar, Ivna. "Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa." Master's thesis, University of Split, School of Medicine, 2020. https://urn.nsk.hr/urn:nbn:hr:171:853736

Ćavar, I. (2020). 'Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa', Master's thesis, University of Split, School of Medicine, accessed 06 May 2024, https://urn.nsk.hr/urn:nbn:hr:171:853736

Ćavar I. Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa [Master's thesis]. Split: University of Split, School of Medicine; 2020 [cited 2024 May 06] Available at: https://urn.nsk.hr/urn:nbn:hr:171:853736

I. Ćavar, "Povezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa", Master's thesis, University of Split, School of Medicine, Split, 2020. Available at: https://urn.nsk.hr/urn:nbn:hr:171:853736

Metadata
TitlePovezanost izražaja hijaluronske kiseline i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa
Title (english)Correlation of expression of hyaluronic acid and its receptor CD44 with the presence of inflammatory infiltrate in gingiva samples of patients diagnosed with advanced generalized periodontitis
AuthorIvna Ćavar
MentorDarko Kero (mentor)
Committee memberVedrana Čikeš Čulić (predsjednik povjerenstva)
Committee memberSnježana Mardešić (član povjerenstva)
Committee memberAntonija Tadin (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Department of Anatomy, Histology and Embryology)
Split
Defense date and country2020, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Dental Medicine
Periodontology
Abstract
Cilj istraživanja Cilj istraživanja bio je ispitati moguću povezanost između izražaja hijaluronske kiseline (HA) i njenog receptora CD44 s prisustvom upalnog infiltrata u uzorcima tkiva gingive pacijenata oboljelih od uznapredovalog generaliziranog parodontitisa. Materijali i metode U svrhu istraživanja metodom imunofluorescencije (IF) obrađeno je 20 uzoraka gingive ispitanika sa zdravim parodontom (kontrole, n = 20) i 20 uzoraka gingive ispitanika s uznapredovalim generaliziranim parodontitisom stadija 3 i 4 (ispitna skupina, n = 20). Za IF bojenje korištena su primarna protutijela na HA i CD44 te na opći biljeg upalnih stanica CD45 za prikazivanje upalnog infiltrata. Preparati su fotografirani tehnikom panoramskog snimanja. Obrada panoramskih snimki provedena je u programu Adobe Photoshop® CC (2019). Izračun izražajnih domena i prostornih gradijenata IF signala napravljen je u programu ImageJ (NIH, Public Domain). Za statističku analizu podataka korišteni su Microsoft Office Excel 2016 (Microsoft Corp, Redmond, WA, USA) i GraphPad v8 (GraphPad Software, La Jolla, CA, USA). Rezultati U uzorcima gingive obje skupine, HA i CD44 izražavaju različite populacije stanica uključujući i upalne stanice. Nije nađena razlika u ukupnom izražaju HA i CD44 u zdravoj i bolesnoj gingivi. Međutim, raspodjela signala na HA i CD44 prema tkivnim odjeljcima (epitel, subepitelna stroma) u bolesnoj gingivi je bila različita što se pokazalo statistički značajnim povećanjem stromalnog izražaja oba ispitivana čimbenika u gingivi ispitanika s uznapredovalim generaliziranim parodontitisom. Ni HA ni CD44 prostorno ne koreliraju s prisustvom upalnog infiltrata što se može objasniti njihovim ubikvitarnim izražajem u gingivi u usporedbi sa znatno ograničenijim izražajem općeg biljega upalnih stanica CD45. Nađena je umjerena međusobna prostorna korelacija izražaja HA i CD44 u bolesnoj gingivi što upućuje na to da CD44 samo djelomično posreduje učinke i/ili sudjeluje u metabolizmu HA u gingivi ispitanika s uznapredovalim generaliziranim parodontitisom. Zaključak Navedeni rezultati ne isključuju ulogu HA i CD44 u regulaciji upalnog odgovora u uznapredovalom generaliziranom parodontitisu, ali potrebna su daljnja istraživanja kako bi se priroda te uloge rasvijetlila.
Abstract (english)
Objectives The aim of the study was to investigate association between the expression of hyaluronic acid (HA) and its main receptor CD44 with the presence of inflammatory infilitrate in gingiva samples of patients suffering from advanced generalized periodontal disease. Material and Methods For this study, 20 samples of gingival tissue from healthy donors (controls, n = 20) and patients suffering from advanced generalized periodontitis stage 3 and 4 (test group, n = 20) were obtained. For the immunofluorescence (IF) staining, primary antibodies against HA, CD44 and general inflammatory cell marker CD45 were used. The microscopic slides were photographed using panoramic technique. Editing of panoramic images was performed in Adobe Photoshop® CC (2019). Quantification of IF signals' expression domains and spatial gradients was performed in ImageJ (NIH, Public Domain). For statistical analysis Microsoft Office Excel 2016 (Microsoft Corp, Redmond, WA, USA) and GraphPad v8 (GraphPad Software, La Jolla, CA, USA) software packages were used. Results In healthy and diseased gingiva, different types of cells (including the inflammatory cells) express HA and CD44. No difference in the total expression of HA and CD44 in healthy and diseased gingiva was found. However, the pattern of distribution of HA and CD44 signals was somewhat different between two groups of samples with regard to increased stromal expression of HA and CD44 in diseased gingiva compared to healthy gingiva. No spatial correlation between HA and CD44 expression with the presence of inflammatory infiltrate was found, which might be explained by the fact that HA and CD44 are ubiquitously expressed in gingival tissue. Moderate spatial correlation between HA and CD44 was found in diseased gingiva, implying its partial involvement in HA signaling and HA metabolism during the advanced generalized periodontitis. Conclusion HA and CD44 might play a role in regulation of inflammatory response in advanced generalized periodontitis. However, further studies are needed in order to properly characterize such role of both investigated factors.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:853736
Study programmeTitle: Dental Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica dentalne medicine (doktor/doktorica dentalne medicine)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-12-03 09:33:19