Objectives: The aim of this study was to asses knowledge, attitudes and habits of medical, dental and pharmacy students about sports supplements and to investigate their relationship with the level of physical activity and the study program. Materials and methods: A cross-sectional survey was conducted using a questionnaire method on students of medicine, dental medicine and pharmacy who attended the University of Split School of Medicine in the academic year 2020/2021. The survey form consisted of two questionnaires - the IPAQ-SF (International Physical Activity Questionnaire - Short Form) and a questionnaire on attitudes and habits about the use of dietary supplements. Students were compared by parameters with regard to the degree of physical activity and the study program. Results: The study included 200 students attending University of Split School of Medicine. Physically more active students used dietary supplements statistically significantly more often compared to less active ones (60.6% vs 43.6%, P=0.023) and achieved a statistically significantly higher number of points on the knowledge questionnaire (7 (6-8) vs 9 (8-10), P <0.001). More than half of the students in both groups stated that they use Internet to search for information on dietary supplements and no statistically significant difference was found between the groups regarding this parameter (P=0.714). Students of all three study programs achieved a similar number of points on the knowledge questionnaire and no statistically significant difference was found between groups (P=0.618). Approximately half of the students in all three study programs stated that they use dietary supplements (medicine 53.2%, dental medicine 53.2%, pharmacy 47.6%). Conclusion: Physical activity is significantly associated with the use of dietary supplements. More physically active students use dietary supplements significantly more and have better knowledge about them, compared to less physically active ones. Students showed insufficient knowledge about sports dietary supplements. It is necessary to increase the education of students about sports supplements within the faculty teaching.