| Abstract | Ciljevi istraživanja: Kvalitativnom metodom (GC-MS) odrediti sastav preparata protiv ispadanja kose te usporediti navode deklaracija s dobivenim rezultatima. Ustroj istraživanja: eksperimentalna studija
Materijali i metode: Ispitana su 2 uzorka preparata protiv ispadanja kose. Za pripremu uzorka korištene su staklene Petrijeve zdjelice na koje je nanesen uzorak volumena 100 μL. Uzorci su ekstrahirani smjesom organskih otapala, kloroforma, etil acetata i N-heksana u jednakom omjeru (v/v/v= 1:1:1), te su potom postavljeni u digestor na sušenje u struji zraka. Kromatografska analiza pripremljenih ekstrakata izvedena je na plinskom kromatografu sa spektrometrom masa, metodom koja omogućava istovremeno snimanje ukupnog ionskog kromatograma (engl. Total ion chromatogram, TIC) u području od 40 – 600 m/z i snimanje samo odabranih iona (engl. Single ion monitoring, SIM).
Rezultati: Kvalitativnom analizom uzoraka dobili smo rezultate koji su izlistani u analitičkom izvješću. U prvom uzorku su pronađene tvari koje nisu navedene među pomoćnim tvarima, ali prema visini signala možemo zaključiti da se radi o tvarima koje se nalaze u tragovima. U trećem uzorku nismo pronašli sve tvari koje proizvođač navodi na ambalaži, a kofein koji je jedan od sastojaka je pokazao jako visok signal što odgovara visokoj koncentraciji.
Zaključak: Ovim istraživanjem je provedena samo kvalitativna analiza te bi za detaljniju obradu dobivenih podataka trebalo provesti i kvantitativnu analizu kako bi u potpunosti točno interpretirali rezultate. |
| Abstract (english) | Objectives: Qualitative determination of ingredients in products for hair loss using GC-MS method, as well as the comparison of the results with those declared on the product.
Design: Experimental study
Material and Methods: Two samples of anti-hair loss products were tested. Petri dishes were used to prepare the sample and volume of 100 μL was applied. Samples were extraced with a mixture of organic solvents, chloroform, ethyl acetate and N-hexane in equal ratio (v / v / v = 1: 1: 1), and then placed in a digester to air dry. Chromatographic analysis of the prepared extracts was performed on a gas chromatograph with a mass spectrometer, a method that allows simultaneous recording of the total ion chromatogram (TIC) in the range of 40 - 600 m / z and recording only selected ions (single ion monitoring, SIM). Results: By qualitative analysis of the samples, we obtained the results listed in the analytical report. In the first sample, substances were found that are not listed among the excipients, but according to the signal height, we can conclude that these are trace substances. In the third sample, we did not find all the substances listed by the manufacturer on the packaging, and caffeine, which is one of the ingredients, showed a very high signal, which corresponds to a high concentration.
Conclusion: With this research, only a qualitative analysis was performed, and for a more detailed processing of the obtained data, a quantitative analysis should be performed in order to fully interpret the results. |
