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master's thesis
Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom
Split: University of Split, School of Medicine, 2021. urn:nbn:hr:171:005628
Perkov-Stipičin, Bruna
University of Split
School of Medicine
Medical chemistry and biochemistry

Institutional repository: MEFST Repository

Cite this document

Perkov Stipičin, B. (2021). Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:005628

Perkov Stipičin, Bruna. "Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:005628

Perkov Stipičin, Bruna. "Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:005628

Perkov Stipičin, B. (2021). 'Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom', Master's thesis, University of Split, School of Medicine, accessed 07 January 2025, https://urn.nsk.hr/urn:nbn:hr:171:005628

Perkov Stipičin B. Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom [Master's thesis]. Split: University of Split, School of Medicine; 2021 [cited 2025 January 07] Available at: https://urn.nsk.hr/urn:nbn:hr:171:005628

B. Perkov Stipičin, "Citotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom", Master's thesis, University of Split, School of Medicine, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:171:005628

Metadata
TitleCitotoksično djelovanje raštike na stanične linije humanih karcinoma mjereno MTT metodom
Title (english)The cytotoxic effect of Brassica oleracea var. acephala on human cancer cell lines measured by MTT method
AuthorBruna Perkov-Stipičin
MentorVedrana Čikeš Čulić (mentor)
Committee memberMaja Valić (predsjednik povjerenstva)
Committee memberMarina Degoricija (član povjerenstva)
Committee memberVedrana Čikeš Čulić (član povjerenstva)
GranterUniversity of Split
School of Medicine
(Medical chemistry and biochemistry)
Split
Defense date and country2021-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Cilj istraživanja je bio ispitati potencijalno citotoksično djelovanje raštike (Brassica oleracea var. acephala) na humane stanice karcinoma dojke (MDA-MB-231) i mokraćnog mjehura (T24). Obje stanične linije tretirane su ekstraktima lista, stabljike i korijena raštike u koncentracijama od 1 µg/mL, 5 µg/mL, 10 µg/mL, 50 µg/mL i 100 µg/mL. Citotoksičnost se određivala nakon 4, 24, 48 i 72 h korištenjem MTT metode. Omjer apsorbancije stanica tretiranih ispitivanim uzorcima te apsorbancije onih koje nisu tretirane pokazatelj je citotoksične aktivnosti korištenih ekstrakata. Najjači citotoksični učinak na stanične linije T24 i MDA-MB-231 imao je ekstrakt korijena u koncentraciji od 50 µg/mL nakon 72h inkubacije. Ispitivani uzorci pokazuju citotoksični učinak ovisan o vremenu inkubacije i koncentraciji. Njihovo djelovanje nije uvijek proporcionalno povećanju koncentracije i vremenu inkubacije, te u pojedinim slučajevima dolazi do povećanja broja metabolički aktivnih stanica. Potencijalan citotoksičan učinak raštike na stanične linije humanih karcinoma, ujedno i hipoteza ovog istraživanja, je potvrđen. Potrebna su daljnja in vivo ispitivanja kako bi se potvrdilo djelovanje i na životinjskim modelima.
Abstract (english)
The aim of the study was to examine the potential cytotoxic effects of Brassica oleracea var. acephala on human breast cancer cells (MDA-MB-231) and bladder (T24). Both cell lines were treated with leaf, stem and root extracts at concentrations of 1 μg/mL, 5 μg/mL, 10 μg/mL, 50 μg/mL and 100 μg/mL. Cytotoxicity was determined after 4, 24, 48 and 72 h using MTT method. The ratio of the absorbance of cells treated with the tested samples and the absorbance of those not treated is an indicator of the cytotoxic activity of the used extracts. The strongest cytotoxic effect on T24 and MDA-MB-231 cell lines had root extract at the concentration of 50 µg/mL after 72 h of incubation. The tested samples showed a cytotoxic effect depended on incubation time and concentration. Their activity is not always proportional to the increase in concentration and incubation time, so in some cases after a certain time there is an increase in the number of metabolically active cells. The potential cytotoxic effect of collard greens on human cancer cell lines, as well as the hypothesis of this study, has been confirmed. Further in vivo studies are needed to confirm the effects in animal models as well.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:005628
ProjectNumber: IP-2016-06-1316
Title: Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnost hiperakumulacije metala
Title: Plants as a source of bioactive sulphur compounds and their ability to hyperaccumulate metals
Acronym: BioSMe
Leader: Ivica Blažević
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-11-08 10:30:07