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master's thesis
Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave
Split: University of Split, School of Medicine, 2021. urn:nbn:hr:171:262323
Radinović, Leah
University of Split
School of Medicine

Institutional repository: MEFST Repository

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Radinović, L. (2021). Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:262323

Radinović, Leah. "Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:262323

Radinović, Leah. "Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave." Master's thesis, University of Split, School of Medicine, 2021. https://urn.nsk.hr/urn:nbn:hr:171:262323

Radinović, L. (2021). 'Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave', Master's thesis, University of Split, School of Medicine, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:262323

Radinović L. Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave [Master's thesis]. Split: University of Split, School of Medicine; 2021 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:171:262323

L. Radinović, "Određivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave", Master's thesis, University of Split, School of Medicine, Split, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:171:262323

Metadata
TitleOdređivanje in vitro i ex vivo gastrointestinalne stabilnosti glukozinolata iz bijele i smeđe gorušice primjenom simuliranog dvofaznog modela probave
Title (english)Determining in vitro and ex vivo gastrointestinal stability of glucosinolates in white and yellow mustard using a simulated two-phase digestion model
AuthorLeah Radinović
MentorTea Bilušić (mentor)
Committee memberSanja Perinović Jozić (predsjednik povjerenstva)
Committee memberFranko Burčul (član povjerenstva)
Committee memberTea Bilušić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2021-10-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Pharmacy
Abstract
Cilj istraživanja bilo je utvrditi stabilnost sinigrina i sinalbina u dvofaznom simuliranom probavnom sustavu. Ispitivani su sinalbin iz bijele gorušice (Sinapis alba L.) i sinigrin iz smeđe gorušice (Brassica juncea L.) koristeći metodu simulacije želučane i crijevne faze. Daljni cilj bio je usporediti stabilnost glukozinolata u odnosu na komercijalne i ljudske enzime u svakoj od dviju faza probave. Za simuliranje ljudske želučane i crijevne faze korišteni su ljudski želučani i crijevni sokovi skupljeni u Kliničkom bolničkom centru Split, a kao komercijalni enzimi korištene su smjese SGF (eng. simulated gastric fluid) za želučanu i SIF (eng. simulated intestinal fluid) za crijevnu fazu. Rezultati su obrađivani na spojenom sustavu HPLC-DAD-MS/MS. Veća stabilnost dokazana je u želučanoj fazi u odnosu na crijevnu fazu za oba glukozinolata. Nadalje, veća stabilnost uočena je prilikom korištenja ljudskih probavnih sokova u usporedbi s komercijalnim smjesama enzima, također za oba glukozinolata i u objema fazama probave. Izlaganje glukozinolata, u ovom slučaju sinigrina i sinalbina, probavnim sokovima dovodi do njihove degradacije i nastajanja razgradnih produkata izotiocijanata. Glukozinolati su podložni razgradnji i u želučanoj i u crijevnoj fazi probave, no veća razdradnja zbiva se u crijevima. Nadalje, komercijalni enzimi u većoj mjeri razgrađuju glukozinolate od ljudskih probavnih enzima, no u obzir se mora uzeti velika interindividualna varijabilnost između ljudskih probavnih sokova.
Abstract (english)
The aim was to determine the stability of sinigrin and sinalbin in a two-phase simulated digestive system. Sinalbin from white mustard (Sinapis alba L.) and sinigrin from yellow mustard (Brassica juncea L.) were studied using a method that simulates the gastric and intestinal phases. Another objective was to compare the stability of glucosinolates in relation to human and commercial enzymes in each of the two digestion phases. To simulate the human gastric and intestinal phases, human gastric and intestinal juices collected from the University Hospital of Split were used. SGF (simulated gastric fluid) for gastric phase and SIF (simulated intestinal fluid) for intestinal phase were used as commercial mixtures. The results were analyzed using HPLC-DAD-MS/MS system. Higher stability was demonstrated in the gastric phase compared to the intestinal phase. Moreover, when human digestive juices were used, higher stability was observed compared to commercial enzyme mixtures for both glucosinolates and in both digestive phases of digestion. When glucosinolates, in this case sinigrin and sinalbin, are exposed to digestive juices, it leads to their degredation and the formation of their degradation products isothiocyanates. Glucosinolates can be degraded in both the gastric and intestinal phase, with degradation being greater in the intestine. In addition, commercial enzymes degrade glucosinolates more than human digestive enzymes, although the large interindividual variability between human digestive juices must be taken into account.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:262323
ProjectNumber: IP-2016-06-1316
Title: Biljke kao izvor bioaktivnih sumporovih spojeva te njihova sposobnost hiperakumulacije metala
Title: Plants as a source of bioactive sulphur compounds and their ability to hyperaccumulate metals
Acronym: BioSMe
Leader: Ivica Blažević
Jurisdiction: Croatia
Funder: HRZZ
Funding stream: IP
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2021-12-22 12:14:57