| Abstract | Cilj istraživanja: Prikaz podataka o broju pozitivnih i negativnih uzoraka prikupljenih u svrhu laboratorijske dijagnostike tuberkuloze u Kliničkom bolničkom centru Split u razdoblju od 01.01.2015. do 31.12.2019. godine. Materijali i metode: U retrospektivnom istraživanju obuhvaćeni su podaci iz arhive Kliničkog zavoda za mikrobiologiju i parazitologiju KBC-a Split i godišnjih publikacija Registra za tuberkulozu Službe za epidemiologiju Hrvatskog zavoda za javno zdravstvo koji analiziraju uzorke upućene na dijagnostiku M.tuberculosisu KBC Split.Obrađeni podaci odnose se na bronhoskopske uzorke, uzorke sputuma, pleuralnihpunktata, likvora, urina, punktata limfnih čvorova, tkiva, briseva rana i punktata kosti. Podaci su obrađeni u programskom paketu Office 2016 (Microsoft, Redmond, WA). Rezultati: Od ukupnog broja testiranih uzoraka, najviše je uzoraka testirano 2015. (N = 2608), a najmanje 2018. godine (N = 2104). Najveći je broj pozitivnih rezultata mikroskopije dobiven 2015. (N = 2114), a najmanji 2017. godine (N = 1771). U skladu s time, kultivacijskom je analizom najveći broj pozitivnih rezultata dobiven 2015. (N = 2608), a najmanji 2017. godine (N = 2104). S obzirom na vrstu uzoraka, najveći je broj testiranih bronhoskopskih uzoraka, zatim slijede uzorci sputuma, urina te pleuralnihpunktata. Uzorci likvora, punktata limfnih čvorova, tkiva te briseva rana u značajno su manjem broju testirani u odnosu na ostale uzorke. Prosjek pozitivnih rezultata mikroskopske i kultivacijske analize uzoraka sputuma i bronhoskopskih uzoraka, kao i kultivacijske analize pleuralnihpunktata, u promatranom razdoblju pokazuje blagi pad. Najveći broj izolataM. tuberculosispotvđen je 2015. (N = 97), a najmanji 2017. godine (N = 45). Testom osjetljivosti na antituberkulotike utvrđena su 4 uzorka s monorezistencijom na izoniazid 2015. godine, dok ostalih godina nisu pronađeni rezistentni uzorci. Rezultati testiranja ukazuju na pad broja oboljelih od tuberkuloze u razdoblju istraživanja. Zaključak: Rezultati istraživanja pokazuju kontinuirani pad u broju osoba zaraženih tuberkulozom u Splitsko-dalmatinskoj županiji tijekom istraživanog razdoblja, kao i u broju dijagnostičkih uzoraka analiziranih u KBC-u Split, što je u skladu s hipotezom istraživačkog rada, kao i s padom incidencije tuberkuloze u Republici Hrvatskoj i u svijetu u promatranom vremenskom razdoblju. |
| Abstract (english) | Objective: Review of the data on the number of positive and negative samples collected for
the purpose of laboratory diagnosis of tuberculosis in the University Hospital of Split in the
period from 01.01.2015. to 31.12.2019.
Materials and methods: The retrospective study processed the data from the archives of the
Clinical Institute of Microbiology and Parasitology of the University Hospital of Split and
annual publications of the Tuberculosis Registry of the Epidemiology Service of the Croatian
Institute of Public Health describing samples collected and analyzed at the University
Hospital of Split for diagnostic confirmation of M. tuberculosis. The processed data refers to
bronchoscopic, sputum, pleural puncture, cerebrospinal fluid, urine, lymph node puncture,
tissue, wound swab and bone puncture samples. The data was processed in the Office 2016
software package (Microsoft, Redmond, WA).
Results: From the total number of tested samples, the most samples were tested in 2015 (N =
2608), and the least in 2018 (N = 2104). The highest number of positive microscopy results
was obtained in 2015 (N = 2114), and the lowest in 2017 (N = 1771). Accordingly, the
highest number of positive cultivation results was obtained in 2015 (N = 2608) and the lowest
in 2017 (N = 2104). Regarding the type of samples, the largest number of tested samples were
bronchoscopic samples, followed by samples of sputum, urine and pleural punctures. Samples
of cerebrospinal fluid, lymph node punctures, tissues and wound swabs were tested in
significantly smaller numbers compared to the other samples. The average of positive results
of microscopic and cultivation analysis of sputum samples and bronchoscopic samples, as
well as cultivation analysis of pleural punctures, shows a slight decrease in the observed
period. The highest number of M. tuberculosis isolates was confirmed in 2015 (N = 97), and
the lowest in 2017 (N = 45). The antituberculosis susceptibility test identified 4 samples with
isoniazid monoresistance in 2015, while no resistant samples were found in the other years.
The test results indicate a decrease in the number of tuberculosis patients during the study
period.
Conclusion: The results of the research show a continuous decrease in the number of persons
infected with tuberculosis in Split-Dalmatia County during the research period, as well as in
the number of diagnostic samples analyzed in KBC Split, which is in line with the research
hypothesis in the observed time period. |
