Objectives: Selenium as well as zinc seem to have an important role in thyroid metabolism as well as in the synthesis of hormones. Autoimmune thyroid diseases are one of the most common thyroidal dysfunctions in iodide sufficient areas. As both trace elements showed beneficial effects in previous studies, we wanted to further investigate this topic. The aim of our study was to elaborate a possible correlation of selenium and zinc with thyroid function tests, TPO-Antibodies as well as the calculated parameter of the secretory capacity of the thyroid gland (SPINA-GT), sum activity of peripheral deiodinases (SPINA-GD), the thyrotroph thyroid hormone sensitivity index (TTSI) and Jostel´s TSH Index (TSHI). Methods: In total, we included 90 patients from the outpatient department of endocrinology of the REGIOMED hospital in Coburg, Germany. The time span of the retrospective study was already collected data from 2015 until November 2017. Blood tests were drawn after a fasting period of at least 12 hours. Body mass index was calculated from height and body weight obtained. Blood pressure and heart rate was measured according to standard. Results: SPINA-GD showed to be not significant lower in the group with high levels of TPO-Antibodies. There was no significant difference neither in regard of the selenium level nor in regard of the level of SPINA-GD between both groups. Selenium showed significant correlation with TSH, TSHI and TTSI in the group of low TPO-Antibodies and with T3 in the group of high TPO-Antibodies. By excluding cases which were additionally treated with T3, neither zinc nor selenium correlated significantly with SPINA-GD. As secondary outcome, selenium correlated significantly with the age and zinc with the heart rate of the participants. Conclusion: People with increased TPO-Antibodies showed lower levels of SPINA-GD, higher levels of TPO-Antibodies and slightly higher levels of selenium. Influences of selenium on thyroid function tests were only in regard of triiodothyronine seen. Further, prospective studies have to be conducted to get more insight into this broad and complex topic of thyroid gland diseases and possible influences of selenium and zinc.