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master's thesis
Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice
Split: University of Split, School of Medicine, 2022. urn:nbn:hr:171:811463
Jerčić, Dora
University of Split
School of Medicine

Institutional repository: MEFST Repository

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Jerčić, D. (2022). Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:811463

Jerčić, Dora. "Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:811463

Jerčić, Dora. "Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice." Master's thesis, University of Split, School of Medicine, 2022. https://urn.nsk.hr/urn:nbn:hr:171:811463

Jerčić, D. (2022). 'Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice', Master's thesis, University of Split, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:811463

Jerčić D. Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice [Master's thesis]. Split: University of Split, School of Medicine; 2022 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:811463

D. Jerčić, "Apoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice", Master's thesis, University of Split, School of Medicine, Split, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:171:811463

Metadata
TitleApoptotsko djelovanje tienopiridinskog derivata na stanice humanog karcinoma grlića maternice
Title (english)Apoptotic effect of thienopyridine derivative on human cervical carcinoma cells
AuthorDora Jerčić
MentorVedrana Čikeš Čulić (mentor)
Committee memberAna Šešelja Perišin (predsjednik povjerenstva)
Committee memberVedrana Čikeš Čulić (član povjerenstva)
Committee memberNikolina Režić Mužinić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2022, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract
Cilj istraživanja: Cilj istraživanja bio je dokazati apoptotsko djelovanje tieno[2,3-b]piridinskog derivata (E)-3-amino-5-(3-(3-bromfenil)akriloil)-N-(3-kloro-2-metilfenil)-6-metiltieno[2,3-b]piridin-2-karboksamida (inhibitor 8) na stanice humanog karcinoma grlića maternice staničnih linija HeLa i SiHa. Materijali i metode: MTT testom provedeno je ispitivanje citotoksičnosti na staničnim linijama HeLa i SiHa te je određena potrebna koncentracija inhibitora 8 kojom je ispitivano njegovo apoptotsko djelovanje. Apoptotski učinak inhibitora 8 na stanične linije HeLa i SiHa praćen je protočnom citometrijom. Rezultati: Prikazani su grafički kao postotak rane i kasne apoptoze i preživjelih stanica. Inhibitor 8 je inducirao ranu apoptozu kod obje ispitivane stanične linije. Nije pokazao značajan učinak na HeLa stanice u kasnoj apoptozi, dok je na SiHa stanice u ranoj apoptozi pokazan najznačajniji učinak. Zaključci: In vitro izlaganje staničnih linija humanog karcinoma grlića maternice, HeLa i SiHa, tieno[2,3-b]piridinskom derivatu dovodi do povećanja postotka stanica u fazi apoptoze. Tretiranje karcinomskih stanica tieno[2,3-b]piridinskim derivatom mogao bi predstavljati novi terapijski pristup liječenja. Potrebna su daljnja in vitro i in vivo ispitivanja na životinjskim modelima kako bi se potvrdilo djelovanje.
Abstract (english)
Objectives: The aim of the research was to prove the apoptotic action of the thieno[2,3-b]pyridine derivative (E)-3-amino-5-(3-(3-bromophenyl)acryloyl)-N-(3-chloro-2- methylphenyl)-6-methylthieno[2,3-b]pyridine-2-carboxamide (inhibitor 8) on human cervical carcinoma cells of HeLa and SiHa cell lines. Materials and methods: The MTT test was used to test cytotoxicity on HeLa and SiHa cell lines, and the required concentration of inhibitor 8 was determined to test its apoptotic effect. The apoptotic effect of inhibitor 8 on HeLa and SiHa cell lines was monitored by flow cytometry. Results: They are shown graphically as a percentage of early and late apoptosis and surviving cells. It can be concluded that inhibitor 8 is effective on both tested cell lines in early apoptosis and in early and late apoptosis overall. It did not show a significant effect on HeLa cells in late apoptosis, while the most significant effect was shown on SiHa cells in early apoptosis. Conclusions: In vitro exposure of human cervical cancer cell lines, HeLa and SiHa, to a thieno[2,3-b]pyridine derivative leads to an increase in the percentage of cells in the apoptotic phase. Treatment of cancer cells with a thieno[2,3-b]pyridine derivative could represent a new therapeutic approach. Further in vitro and in vivo studies with animal models are required to confirm the effect.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:811463
Study programmeTitle: Pharmacy
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-09-27 09:46:39