prikaz prve stranice dokumenta Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa
  Download
PDF 1.61 MB
doctoral thesis
Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa
Split: University of Split, School of Medicine, 2019. urn:nbn:hr:171:760372
Alujević-Jakus, Ivana
University of Split
School of Medicine

Institutional repository: MEFST Repository

Cite this document

Alujević Jakus, I. (2019). Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:760372

Alujević Jakus, Ivana. "Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa." Doctoral thesis, University of Split, School of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:171:760372

Alujević Jakus, Ivana. "Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa." Doctoral thesis, University of Split, School of Medicine, 2019. https://urn.nsk.hr/urn:nbn:hr:171:760372

Alujević Jakus, I. (2019). 'Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa', Doctoral thesis, University of Split, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:760372

Alujević Jakus I. Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa [Doctoral thesis]. Split: University of Split, School of Medicine; 2019 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:760372

I. Alujević Jakus, "Disfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa", Doctoral thesis, University of Split, School of Medicine, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:171:760372

Metadata
TitleDisfunkcija mitohondrija i stanični odgovor na hipoksiju u fibromuskularnim strukturama DNA male zdjelice u žena s prolapsom zdjeličnih organa
Title (english)Mitochondrial dysfunction and cellular response to hypoxia in fibromuscular structures of the pelvic floor in women with pelvic organ prolapse
AuthorIvana Alujević-Jakus
MentorKatarina Vilović (mentor)
Committee memberMarko Ljubković (predsjednik povjerenstva)
Committee memberTomislav Strinić (član povjerenstva)
Committee memberSnježana Tomić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2019, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Gynecology and Obstetrics
Universal decimal classification
(UDC)		618 - Gynaecology. Obstetrics
Abstract
Cilj istraživanja: Cilj ovog istraživanja je istražiti postoji li stanični odgovor na hipoksijsko oštećenje u stanicama zdjeličnih struktura žena s POP-om (prolapsom zdjeličnih organa) i žena iz kontrolne skupine te analizirati mitohondrijsku funkciju u mišićnom sloju rodnice žena s POP-om i usporediti je s uzorcima rodnice žena iz kontrolne skupine. Materijali i metode: U istraživanje je uključeno 120 ispitanica hospitaliziranih u Klinici za ženske bolesti i porode KBC Split. Ispitanice su podijeljene u dvije skupine. POP skupinu (60 ispitanica) činile su pacijentice koje su pristupile vaginalnoj histerektomiji zbog POP-a. Kontrolnu skupinu (60 ispitanica) činile su bolesnice u kojih je izvršena abdominalna histerektomija zbog dobroćudnih ginekoloških bolesti. Analiza mitohondijske funkcije napravljena je za dodatnih 16 ispitanica (10 u POP skupini, a 6 u kontrolnoj skupini). Tijekom operacijskog zahvata izvršeno je uzorkovanje tkiva. Imunohistokemijskim metodama određena je pozitivnost proteina HIF-1α u stanicama rodnice, uterosakralnog ligamenta i analnog levatora. Metodom western blotting određen je status pet respiracijskih kompleksa mitohondrija u mišićnom sloju rodnice. Rezultati: U bolesnica s POP-om pronađen je veći broj HIF-1α pozitivnih stromalnih stanica u stijenci vagine u odnosu na kontrolnu skupinu (P < 0,05), dok nije postojao statistički značajan porast ekspresije HIF-1α u uterosakralnom ligamentu i analnom levatoru kod žena s prolapsom u usporedbi s kontrolnom skupinom. U bolesnica s POP-om pronađena je smanjena ekspresija mitohondrijskog respiratornog kompleksa II u vaginalnoj stijenci u usporedbi s kontrolnom skupinom (P < 0,05). Zaključak: Rezultati ukazuju na postojanje hipoksijskog oštećenja i poremećenog mitohondrijskog oksidativnog kapaciteta u žena s POP-om u usporedbi s kontrolnom skupinom, što bi moglo biti važno u pojašnjavanju etiopatogeneze POP-a.
Abstract (english)
Objective: The objective of this study was to investigate whether or not there is a cellular response to hypoxic damage in cells of pelvic structures in women with POP (pelvic organ prolapse) and in those from the control group, as well as to analyze the mitochondrial function in the muscular layer of the vagina in woman with POP, and to compare it with the samples collected from the control group. Materials and Methods: The study included 120 subjects hospitalized at the Department of Gynecology and Obstetrics, University Hospital Center of Split. The subjects were divided into two groups. The POP group (60 subjects) consisted of patients who underwent vaginal hysterectomy due to POP. The control group (60 respondents) consisted of patients who underwent abdominal hysterectomy due to benign gynecological diseases. Mitochondrial function analysis was performed for 16 subjects (10 from the POP group and 6 from the control group). During the surgical procedure, tissue sampling was performed. Immunohistochemical methods were used to determine the positivity of the HIF-1α protein in vaginal cells, uterosacral ligaments and levator ani muscle. The Western blotting method was used to determine the status of five respiratory mitochondria complexes in the muscle layer of the vagina. Results: A higher number of HIF-1α positive stromal cells was found in the vaginal wall of women with POP in comparison with those from the control group (P <0.05), while there was no statistically significant increase in expression of HIF-1α in the uterosacral ligament or levator ani muscle of women with prolapse in comparison with those from the control group. A decreased expression of mitochondrial respiratory complex II was found in the vaginal wall of patients with POP in comparison with those from the control group (P <0.05). Conclusion: Results indicate the existence of hypoxic damage and dysfunctional mitochondrial oxidative capacity in women with POP in comparison with the control group, which might be of importance when it comes to clarifying POP etiopathogenesis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:760372
Study programmeTitle: Basic and clinical medical sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti (doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-11 07:17:48