prikaz prve stranice dokumenta Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima
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doctoral thesis
Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima
Split: University of Split, School of Medicine, 2017. urn:nbn:hr:171:303336
Đevenica, Danka
University of Split
School of Medicine

Institutional repository: MEFST Repository

Cite this document

Đevenica, D. (2017). Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:303336

Đevenica, Danka. "Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima." Doctoral thesis, University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:171:303336

Đevenica, Danka. "Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima." Doctoral thesis, University of Split, School of Medicine, 2017. https://urn.nsk.hr/urn:nbn:hr:171:303336

Đevenica, D. (2017). 'Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima', Doctoral thesis, University of Split, School of Medicine, accessed 22 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:303336

Đevenica D. Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima [Doctoral thesis]. Split: University of Split, School of Medicine; 2017 [cited 2024 December 22] Available at: https://urn.nsk.hr/urn:nbn:hr:171:303336

D. Đevenica, "Analiza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima", Doctoral thesis, University of Split, School of Medicine, Split, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:171:303336

Metadata
TitleAnaliza leukocitnih biljega CD15s i CD11b u krvi štakora, te glikosfingolipida CD77 i glikofosfoproteina CD34 u bubrezima, srcu i plućima štakora u hiperbaričnim uvjetima
Title (english)Analysis of leukocyte markers CD15s and CD11b in the blood of rats, and glycosphingolipid CD77 and glycophosphoprotein CD34 in the kidneys, heart and lungs of rats under hyperbaric conditions
AuthorDanka Đevenica
VIAF: 305684333
MentorVedrana Čikeš Čulić (mentor)
Committee memberMirna Saraga-Babić (predsjednik povjerenstva)
Committee memberInes Drenjančević (član povjerenstva)
Committee memberDragan Ljutić (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2017, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Clinical Biochemistry
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Uvod: Cilj ovog istraživanja bio je odrediti učinak hiperbaričnog tretmana (HB) na leukocitne proupalne biljege CD15s i CD11b. Dodatno, istraživanje opisuje promjene u izražaju CD77 i CD34 biljega na stanicama bubrežnog, plućnog i srčanog tkiva nakon hiperbaričnog tretmana. Materijali i metode: Izražaj CD11b i CD15s na leukocitima, kao i CD77 i CD34 biljega na suspenziji stanica bubrežnog, plućnog i srčanog tkiva u životinja nakon hiperbaričnog tretmana i u kontrolnim životinjama, određen je protočnom citometrijom. Rezultati: Hiperbarični tretman je statistički značajno povećao postotak leukocitnih CD15s+CD11b- stanica (od 1.71 ± 1.11 do 23.42 ± 2.85, P < 0.05). Hiperbarični tretman je statistički značajno smanjio postotak CD77+CD34- i CD77+CD34+ stanica u bubrežnom tkivu (od 16.35 ± 5.5 do 4.48 ± 1.28, P < 0.05). Hiperbarični tretman je statistički značajno povećao postotak CD34+ stanica u plućnom tkivu (od 3.27 ± 2.01 do 11.92 ± 6.22, P < 0.05). Naše istraživanje je prvo koje je pokazalo utjecaj hiperbaričnog tretmana na CD34+ stanice u srčanom tkivu kod štakora. Zaključak: Naši rezultati povećanog postotka endotelnog liganda CD15s+ na leukocitima, nakon hiperbaričnog tretmana, upućuju na njegovu ulogu u procesima oštećenja endotela. Pronaši smo također značajni porast postotka CD34+ stanica u srčanom tkivu kao i postotka CD34+ stanica u plućnom tkivu nakon hiperbaričnog tretmana, što može biti posljedica mehanizama oporavka oštećenog endotela usljed hiperoksije.
Abstract (english)
Objective(s): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression. In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure. Materials and Methods: Expression of CD11b and CD15s on leukocytes, as well as CD77 and CD34 expression on endothelial cells in cell suspensions of renal, pulmonary and cardiac tissue in rats after hyperbaric treatment and in control rats were determined by flow cytometry. Results: Hyperbaric treatment significantly increased percentage of leukocytes expressing CD15s+CD11b- (from 1.71 ± 1.11 to 23.42 ± 2.85, P < 0.05). Hyperbaric treatment significantly decreased sum percentage of CD77+CD34- and CD77+CD34+ renal cells (from 16.35 ± 5.5 to 4.48 ± 1.28, P < 0.05). Hyperbaric treatment significantly increased percentage of CD34+ pulmonary cells (from 3.27 ± 2.01 to 11.92 ± 6.22, P < 0.05). Our study is the first reporting the hyperbaric environment influence on CD34+ heart cells in rats. Conclusion: The current findings of increased percentage of leukocytes expressing endothelial selectin ligand CD15s after hyperbaric treatment, point its role in endothelial damage. We found out a significantly increase in percentage of CD34+ cardiac cells as well as CD34+ pulmonary cells in rats after HB treatment which could be a part of repair mechanism of injured endothelium caused by hyperoxia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:303336
Study programmeTitle: Biology of Neoplasms
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-12 12:15:35