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doctoral thesis
Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora
Split: University of Split, School of Medicine, 2015. urn:nbn:hr:171:465535
Režić Mužinić, Nikolina
University of Split
School of Medicine

Institutional repository: MEFST Repository

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Režić Mužinić, N. (2015). Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:465535

Režić Mužinić, Nikolina. "Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora." Doctoral thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:465535

Režić Mužinić, Nikolina. "Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora." Doctoral thesis, University of Split, School of Medicine, 2015. https://urn.nsk.hr/urn:nbn:hr:171:465535

Režić Mužinić, N. (2015). 'Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora', Doctoral thesis, University of Split, School of Medicine, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:465535

Režić Mužinić N. Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora [Doctoral thesis]. Split: University of Split, School of Medicine; 2015 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:465535

N. Režić Mužinić, "Hiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora", Doctoral thesis, University of Split, School of Medicine, Split, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:171:465535

Metadata
TitleHiperkalcijemija uzrokuje proupalni fenotip leukocita i endotelnih stanica štakora
Title (english)Hypercalcemia induces a proinflammatory phenotype in rat leukocytes and endothelial cells
AuthorNikolina Režić Mužinić
MentorVedrana Čikeš Čulić (mentor)
Committee memberJanoš Terzić (predsjednik povjerenstva)
Committee memberDrago Batinić (član povjerenstva)
Committee memberIvana Novak Nakir (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2015, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Basic Medical Sciences
Medical Biochemistry
Universal decimal classification
(UDC)		61 - Medical sciences
Abstract
Mikrodomene endotelnih plazma membrana, takozvane lipidne splavi/kaveole, bogate su neutralnim glikosfingolipidima, globotriozilceramidom Gb3Cer ili CD77. Nekoliko membranskih Ca2+ kanala i pumpi nađeni su u lipidnim splavima/kaveolama. Povećani unos Ca2+ mogao bi uzrokovati endotelni proupalni fenotip. Zbog toga je cilj ove studije odrediti utjecaj hiperkalcijemije u štakora određivanjem izražaja CD77 na CD34+ endotelnim stanicama srca, bubrega i vene kave. Potencijalni proupalni fenotip uzrokovan kalcijem određen je izražajem CD11b i CD15s na leukocitima. Da bi se uzrokovala hiperkalcijemija, Spraque-Dawley mužjacima štakora davana je 1,5% otopina CaCl2 tijekom 14 dana. Izražaj CD77 na CD34+ endotelnim stanicama stanične suspenzije srca, bubrega i vene kave, te izražaj leukocitnih CD11b i CD15s u hiperkalcijemijskoj i kontrolnoj skupini štakora određeni su protočnim citometrom. Koncentracija ioniziranog kalcija u plazmi je bila 1,37 ± 0,01 mM u hiperkalcijemijskoj naspram 1,19 ± 0,03 mM u kontrolnoj skupini štakora. Skupina s hiperkalcijemičnim štakorima pokazala je statistički značajno niži postotak CD34+CD77 stanica u bubregu i veni kavi uz istodobno povećanje postotka leukocita koji imaju izražene proupalne biljege CD11b i CD15s. Pretpostavlja se da su plazma membrane CD77 endotelnih stanica siromašnije u lipidnim mikrodomenama, a time slabije u kontroli Ca2+ izljeva. Postotak CD11b+CD15s+ leukocita mogao bi biti mjera proupalnog učinka kod blage hiperkalcijemije.
Abstract (english)
Endothelial plasma membrane lipid microdomains, so called lipid rafts/caveolae, are rich in neutral glycosphingolipid, globotriaosylceramide, Gb3Cer or CD77. Several plasma membrane Ca2+ channels and pumps are located in lipid rafts/caveolae. Increased Ca2+ influx could cause the development of an endothelial proinflammatory phenotype. Therefore, the aim of this study was to estimate effects of hypercalcemia in rats by determination of CD77 expression on CD34+ endothelial cells in heart, kidney and vena cava. In addition, potential proinflammatory calcium effect was estimated by CD11b and CD15s expression on leukocytes. To achieve hypercalcemia, Sprague-Dawley male rats were drinking CaCl2 solution with a concentration of 1.5% elemental calcium during 14 days. CD77 expression on CD34+ endothelial cells in cell suspensions of heart, kidney and vena cava, as well as leukocyte expression of CD11b and CD15s in hypercalcemic and control rats were determined by flow cytometry. Ionized calcium concentration in plasma was 1.37  0.01 mM in hypercalcemic vs. 1.19  0.03 mM in control rats. Hypercalcemic group showed statistically significantly decreased proportion of endothelial CD34+CD77- cells in the kidney and vena cava in parallel with increase of percentage of leucocytes expressiong proinflammatory markers CD11b and CD15s. In conclusion, it is tempting to speculate that plasma membranes of glycosphingolipid CD77- endothelial cells are poorer in caveolae lipid microdomains and therefore weaker in controlling of Ca2+ influx. The percentage of CD11b+CD15s+ leukocytes could be a measure of proinflammatory effects of mild hypercalcemia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:465535
Study programmeTitle: Biology of Neoplasms
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstvo (doktor/doktorica znanosti, područje biomedicine i zdravstvo)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-15 10:33:19