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doctoral thesis
Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora
Split: University of Split, School of Medicine, 2014. urn:nbn:hr:171:609964
Todorić, Davor
University of Split
School of Medicine

Institutional repository: MEFST Repository

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Todorić, D. (2014). Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:609964

Todorić, Davor. "Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora." Doctoral thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:609964

Todorić, Davor. "Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora." Doctoral thesis, University of Split, School of Medicine, 2014. https://urn.nsk.hr/urn:nbn:hr:171:609964

Todorić, D. (2014). 'Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora', Doctoral thesis, University of Split, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:609964

Todorić D. Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora [Doctoral thesis]. Split: University of Split, School of Medicine; 2014 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:609964

D. Todorić, "Otvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora", Doctoral thesis, University of Split, School of Medicine, Split, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:171:609964

Metadata
TitleOtvorena biopsija testisa štakora prije puberteta, njen utjecaj na spermatogenezu i serumske razine folikulo stimulirajućeg hormona i inhibina B u odrasloj dobi štakora
Title (english)Open biopsy of rat testis before puberty, its influence on spermatogenesis and serum levels of follicle-stimulating hormone and inhibin B in adult rats
AuthorDavor Todorić
MentorIvo Jurić (mentor)
Committee memberMarijan Šitum (predsjednik povjerenstva)
Committee memberMarijana Peruzović (član povjerenstva)
Committee memberStipe Botica (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2014, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Pozadina/ Svrha: Otvorena biopsija testisa jedan je od načina koji omogućuje precizno odrediti buduću plodnost nespuštenog testisa. Cilj ovog istraživanja je bio utvrditi posljedice otvorene biopsije testisa na njihovu buduću plodnost u odrasloj dobi. Metode: Trideset osam mužjaka štakora soja Sprague Dawley u dobi od 20 dana je podijeljeno u tri skupine ovisno o izvršenom zahvatu. Prva skupina je bila kontrolna skupina, druga skupina je bila skupina lažno operiranih, a treća skupina su bili štakori kojima je učinjena otvorena biopsija lijevog testisa. Sedamdesetog dana života nakon obostrane orhidektomije svim skupinama štakora određene su serumske vrijednosti inhibina B i FSH. Odstranjene testise smo uspoređivali obzirom na masu, volumen, spermatogenezu, promjene u histološkoj slici i broju apoptotskih stanica u oba testisa, te promjene serumskih razina inhibina B i FSH među skupinama. Rezultati: U skupini štakora s učinjenom otvorenom biopsijom testisa došlo je do smanjenja mase, volumena i spermatogeneze u bioptiranom testisu sa smanjanjem broja seminifernih tubula, njihovog promjera i debljine zametnog epitela. Također u bioptiranoj skupini testisa dogodio se porast apoptotskog indeksa bez kompenzatorne hipertrofije suprotnog testisa. Nismo dokazali statistički značajnu razliku serumskih razina inhibina B i FSH među ispitivanim skupinama. Zaključak: Otvorena biopsija testisa prije puberteta u štakora ima štetan utjecaj na buduću plodnost štakora u odrasloj dobi. Oštećenje jednog testisa izazvanog otvorenom biopsijom testisa ne uzrokuje kompenzatornu hipertrofiju suprotnog testisa niti dovodi do poremećaja u lučenju i serumskih razina inhibina B i FSH.
Abstract (english)
Background/ Purpose: Open testis biopsy (OTB) is one of the options to accurately assess fertility potential of the undescended testis. The aim of the study was to investigate consequences of OTB in prepubertal rats on their adulthood fertility. Methods: Thirty-eight Sprague Dawley prepubertal male rats were divided into three groups depending on 20th day procedure. The first group was the control group, the second sham operated and the third has left OTB. Bilateral orchidectomy was performed on 70th day to all groups, with determination of serum inhibin B and Follicle stimulating hormone (FSH). Removed testicles have been compared according to the weight, volume, spermatogenesis, histological and apoptotic changes in both testicles with differences in serum levels of inhibin B and FSH. Results: Ipsilateral testis weight, volume, and spermatogenesis reduction with a reduction of tubular number, diameter and germinative epithelium was found in OTB group. Significant increase in apoptotic index was found in biopsied testis without compensatory hypertrophy of contralateral testis. Differences of inhibin B and FSH were not statistically significant among three groups. Conclusion: OTB in prepubertal rats has detrimental effects on fertility in adulthood. It does not cause compensatory hypertrophy of the contralateral testis or disturb serum levels of inhibin B and FSH.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:609964
Study programmeTitle: Basic and clinical medical sciences
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti (doktor/doktorica znanosti, područje biomedicine i zdravstva, polje kliničke medicinske znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-06-02 07:23:51