| Abstract | Cilj: Istražiti vremensku i prostornu raspodjelu epitelnog membranskog antigena (EMA),
mezotelina i nestina u seroznim ovojnicama u fetusa i u odraslih ljudi, kao i u tumorima
mezoteliomima te utvrditi postoje li u navedenim tkivima stanice reaktivne na navedene
biljege.
Materijal i metode: Parafinski rezovi seroznih ovojnica pleure i perikarda sakupljeni su od 6
ljudskih fetusa starosti 15. -22. tjedana, 6 uzoraka pleure i perikarda u odraslih i 6 uzoraka
epiteloidnih mezotelioma i obojani hemalaunom i eozinom te imunohistokemijskim
tehnikama na biljege za EMA, mezotelin i nestin. Imuno-lokalizacija korištenih biljega je
analizirana primjenom semi-kvantitativne metode i statističkih metoda, korištenjem
statističkog programa GraphPad verzija 3.0. te Kruskal–Wallis i Dunnovog posthoc testa.
Rezultati: U seroznim ovojnicama, EMA se pojavio u fetalnom mezotelu, a kasnije se zadržao
samo u mezotelu perikarda, te mezotelu i vezivnom tkivu pleure. Mezotelin se prvi put
pojavio u submezotelnim stanicama perikarda, a u pleuri odmah u mezotelu, dok su u odraslih
EMA i mezotelin bili izraženi samo u površinskom mezotelu. Tijekom razvoja, nestin je bio
izražen u vezivnom tkivu i stanicama miokarda, dok se nakon rođenja njegov izražaj zadržao
samo u endotelnim stanicama krvnih žila.
Zaključak: Tijekom normalnog razvoja seroznih ovojnica i tumorogeneze koja vodi nastanku
mezotelioma, nestin je bio svojstven nezrelim stanicama, dok su EMA i mezotelin bili
izraženi u zrelim epitelnim (mezotelnim) stanicama. Submezotelne stanice perikarda i
mezotelne stanice pleure vjerojatno pripadaju populaciji matičnih stanica. Neoplastična
pretvorba ovih staničnih linija može dovesti do nastanka mezotelioma. |
| Abstract (english) | Aim: To investigate temporal and spatial distribution of epithelial membrane antigen (EMA),
mesothelin and nestin in serous membranes of fetuses, adult humans and tumors
mesotheliomas, and to establish whether cells reactive to those markers exist within tissue
samples.
Materials and methods: Paraffin sections of serous membranes pleura and pericardium,
which were collected from 6 human fetuses 15.-22. weeks old, 6 samples of adult pleura and
pericardium, and 6 samples of epitheloid mesothelioma, were stained with haematoxylin and
eosin, and immunohistochemically with EMA, mesothelin and nestin markers. Immunolocalization
of the three markers was analyzed by using semi-quantitative methods and
statistic methods, applying statistic program GraphPad version 3.0 and Kruskal-Wallis and
Dunn’s posthoc test.
Results: In serous membranes, EMA first appeared in fetal mesothelium, while later on it
retained reactivity only in pericardial mesothelium, as well as in mesothelium and connective
tissue of pleura. Mesothelin first appeared in submesothelial cells of pericardium, within
pleura it appeared in mesothelium, while in adults EMA and mesothelin were expressed only
in surface mesothelium. During development, nestin was expressed in connective tissue and
myocardial cells, while after birth its expression remained only in endothelial cells of blood
vessels.
Conclusions: During normal development of serous membranes and tumorogenesis leading to
appearance of mesothelioma, nestin characterized immature cells, while EMA and mesothelin
were expressed in mature epithelial (mesothelial) cells. Submesothelial cells of pericardium
and pleural mesothelial cells probably belong to the population of immature (stem) cells.
Neoplastic transformation of those cell lineages can lead to genesis of mesothelioma. |
