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doctoral thesis
Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline
Split: University of Split, School of Medicine, 2012. urn:nbn:hr:171:848709
Rogulj, Dinko
University of Split
School of Medicine

Institutional repository: MEFST Repository

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Rogulj, D. (2012). Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline (Doctoral thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:848709

Rogulj, Dinko. "Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline." Doctoral thesis, University of Split, School of Medicine, 2012. https://urn.nsk.hr/urn:nbn:hr:171:848709

Rogulj, Dinko. "Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline." Doctoral thesis, University of Split, School of Medicine, 2012. https://urn.nsk.hr/urn:nbn:hr:171:848709

Rogulj, D. (2012). 'Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline', Doctoral thesis, University of Split, School of Medicine, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:171:848709

Rogulj D. Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline [Doctoral thesis]. Split: University of Split, School of Medicine; 2012 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:171:848709

D. Rogulj, "Usporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline", Doctoral thesis, University of Split, School of Medicine, Split, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:171:848709

Metadata
TitleUsporedba pokazatelja metaboličkog sindroma : uloga oksidativnog oštećenja deoksiribonukleinske kiseline
Title (english)Comparison of the metabolic syndrome determinants : the role of the oxidative DNA damage
AuthorDinko Rogulj
MBZ: 226205
MentorSpomenka Ljubić (mentor)
Committee memberMaja Radman (predsjednik povjerenstva)
Committee memberDavorka Sutlović (član povjerenstva)
Committee memberStjepan Gamulin (član povjerenstva)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2012, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Universal decimal classification
(UDC)		616 - Pathology. Clinical medicine
Abstract
Oksidativni stres sudjeluje u nastanku metaboličkog sindroma i razvoju njegovih komplikacija. Cilj našeg istraživanja bio je ustanoviti postoji li značajnije oksidativno oštećenje DNA i lipida u ispitanika srednje životne dobi s metaboličkim sindromom. Također smo željeli naći najbolji rani pokazatelj metaboličkog sindroma. Usporedili smo pokazatelje općeg i oksidativnog oštećenja DNA, malondialdehid kao pokazatelj peroksidacije lipida, reducirani glutation kao pokazatelj antioksidativnog statusa, antropometrijske, biokemijske i hematološke pokazatelje između skupine ispitanika s metaboličkim sindromom (69 ispitanika) i kontrolne skupine (59 ispitanika). Metodama rekurzivnog particioniranja, uporabom klasifikacijskih algoritama REPTree i Simple CART željeli smo izdvojiti najbolji pokazatelj metaboličkog sindroma. Dvije skupine značajno su se razlikovale u svim antropometrijskim, općim biokemijskim i hematološkim pokazateljima, osim u broju neutrofilnih granulocita i razini fibrinogena. Pokazatelji oštećenja DNA, razine malondialdehida i glutationa nisu se značajno razlikovali između dviju skupina. Dva različita postupka rekurzivnog particioniranja izdvojila su indeks masne jetre kao najbolji pokazatelj koji razdvaja ispitanike s metaboličkim sindromom od ispitanika kontrolne skupine (Weka REPTree 67,82, SimpleCART 70,56). ROC analiza ustanovila je visoku pouzdanost indeksa masne jetre kao pokazatelja koji definira metabolički sindrom. U ispitanika srednje životne dobi s metaboličkim sindromom nismo našli znakove oštećenja jezgrene DNA i lipida. Istraživanje je ukazalo na indeks masne jetre kao najbolji pokazatelj metaboličkog sindroma.
Abstract (english)
Oxidative stress is involved in the genesis of the metabolic syndrome and its complications. The aim of our study was to examine significant oxidative damage of DNA and lipids in middle-aged subjects with metabolic syndrome. Also, we intended to find the best early indicator of metabolic syndrome. We compared markers of primary and specific oxidative damage of DNA, malondialdehyde as marker of lipid peroxidation, and reduced glutathione as marker of antioxidative status, anthropometric, biochemical and hematological parameters between subjects with metabolic syndrome (n=69) and healthy controls (n=59). We used methods of recursive partitioning, Weka REPTree and SimpleCART algorithms to identify the most reliable predictor of metabolic syndrome. Our study has shown a significant difference between healthy individuals and MetS patients in anthropometric, biochemical, and hematological parameters (save for fibrinogen and neutrophils). Oxidative damage parameters of DNA, levels of malondialdehyde and glutathione did not differ between the groups. Two different methods of recursive partitioning signed out fatty liver indeks as the best parameter for discriminating between healthy and metabolic syndrome subjects (Weka REPTree 67,82, SimpleCART 70,56). This finding was confirmed by the receiver-operating curve (ROC) analysis as the best discriminating value for metabolic syndrome. The signs of oxidative damage of DNA and lipids were absent in middle-aged subjects with metabolic syndrome. Our study has indicated the fatty liver index as the best indicator of the metabolic syndrome.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:848709
Study programmeTitle: Obtaining a doctorate of science outside of doctoral studies
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-04-15 12:08:27