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master's thesis
Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga
Split: University of Split, School of Medicine, 2012. urn:nbn:hr:171:852036
Šamanović, Ljubomir
University of Split
School of Medicine

Institutional repository: MEFST Repository

Cite this document

Šamanović, Lj. (2012). Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga (Master's thesis). Split: University of Split, School of Medicine. Retrieved from https://urn.nsk.hr/urn:nbn:hr:171:852036

Šamanović, Ljubomir. "Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga." Master's thesis, University of Split, School of Medicine, 2012. https://urn.nsk.hr/urn:nbn:hr:171:852036

Šamanović, Ljubomir. "Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga." Master's thesis, University of Split, School of Medicine, 2012. https://urn.nsk.hr/urn:nbn:hr:171:852036

Šamanović, Lj. (2012). 'Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga', Master's thesis, University of Split, School of Medicine, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:171:852036

Šamanović Lj. Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga [Master's thesis]. Split: University of Split, School of Medicine; 2012 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:171:852036

Lj. Šamanović, "Povezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga", Master's thesis, University of Split, School of Medicine, Split, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:171:852036

Metadata
TitlePovezanost polimorfizma RS1799983 gena endotelne sintaze dušikova monoksida s perinatalnim hipoksično-ishemičnim oštećenjem mozga
AuthorLjubomir Šamanović
MentorTatijana Zemunik (mentor)
GranterUniversity of Split
School of Medicine
Split
Defense date and country2012, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Gynecology and Obstetrics
Abstract
CILJ ISTRAŽIVANJA: Istražiti moguću povezanost polimorfizma rs1799983 NOS3 gena s razvojem hipoksičnoishemijske encefalopatije (HIE). USTROJ ISTRAŽIVANJA: Studija genetske povezanosti (engl. association study), populacijska studija ispitanika i kontrola (engl. population-based case-control study). Ispitanici su podijeljeni u dvije skupine po 70, ispitivanu i kontrolnu. Svim ispitanicima su uzeti uzorci krvi nakon čega je izolacijom DNA s pomoću QIAamp DNA Blood Mini Kit-ova i metodom analize PCR-om u stvarnom vremenu, određeno prisustvo polimorfizma rs1799983 NOS3 gena. MJESTO ISTRAŽIVANJA: Klinika za dječje bolesti KBC-a Split i Laboratorij za medicinsku biologiju Medicinskog fakulteta u Splitu. SUDIONICI: U istraživanju je sudjelovalo 155 ispitanika. U kontrolnoj skupini bilo je 70 zdrave djece. U ispitivanoj skupini bilo je 85 djece s HIE. Petnaest članova ispitivane skupine isključeno je iz istraživanja. GLAVNE MJERE ISHODA: Statistički značajna korelacija funkcionalnog polimorfizma rs1799983 gena NOS3 i nastanka hipoksično-ishemičnog oštećenja mozga. REZULTATI: Nismo utvrdili postojanje alelske(χ2 =0.016, P=0.897) niti genotipske(χ2=0,331823, P=0,847121) povezanosti polimorfizma rs1799983 gena NOS3 s hipoksično-ishemijskom encefalopatijom. ZAKLJUČAK: Nema povezanosti polimorfizma rs1799983 NOS3 gena s razvojem hipoksično-ishemijske encefalopatije.
Abstract (english)
OBJECTIVE: Explore the possible correlation of the NOS3 gene polymorphism 1799983 with development of hypoxic-ischemic encephalopathy (HIE). DESIGN: Association study, population-based case-control study. Participants were divided into two groups counting 70 people, test group and control group. Blood samples were taken from all participants. DNA was isolated using QIAamp DNA Blood Mini Kit and NOS3 gene polymorphism rs1799983 was determined using real time PCR. SETTINGS: The research was conducted at the Clinic for children diseases of the Clinical Hospital Centre in Split and laboratory of Medical Biology School of Medicine, Split. PARTICIPANTS: Participants were divided into two groups. Control group counted 70 healthy children. Test group had 85 children with diagnosed hypoxic-ischemic encephalopathy. Fifteen participants were taken out from the test group during the research. MAIN OUTCOME MEASURE: Statistical correlation between functional polymorphism rs1799983 of NOS3 gene and hypoxicischemic brain damage was not found. RESULTS: We have not determined allelic(χ2 =0.016, P=0.897) or genotype(χ2=0,331823, P=0,847121) correlation between polymorphism rs1799983 of NOS3 gene and hypoxic-ischemic encephalopathy. CONCLUSION: There is no genetic effect of NOS3 gene polymorphism rs1799983 on development of hypoxicischemic encephalopathy.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:171:852036
Study programmeTitle: Medicine
Study programme type: university
Study level: integrated undergraduate and graduate
Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2016-05-16 10:54:37