Objective of the Study: The primary objective of this study was to determine the association between chronic cannabidiol supplementation and parameters of glucose metabolism in patients with prediabetes. Participants and Procedures: The study included 37 participants with prediabetes. As part of the research, a clinical examination and anthropometric measurement were performed on each subject along with taking a medical history. During each experimental visit, a venous blood sample was taken from the cubital vein and a urine sample was taken. After the initial blood sample was taken, subjects were assessed using a bioelectrical impedance scale. During 12-week period, the subjects received CBD (5 weeks) and Placebo (5 weeks) with a two-week washout between dosing periods. After each dosing period, the subjects came back to the laboratory where all anthropometric measurements, blood pressure measurements, blood and urine sampling were repeated. Results: Following a 12-week intervention (5 weeks of CBD and 5 weeks of placebo, with a two-week washout), there was a statistically significant decrease in fasting plasma glucose (5.3 ± 0.6 mmol/L vs. 5.1 ± 0.5 mmol /L, P = 0.029). On the other hand, as a result of the same intervention, there was no significant reduction in HbA1c (5.8 ± 0.2 % vs. 5.9 ± 0.2 %, P = 0.148) or blood insulin (10.5 (7.1 – 13.5) μU/mL vs. 10.7 (7.5 – 16.0) μU/mL, P = 0.976). Finally, there was no significant dynamics in LDL-cholesterol concentrations (3.4 ± 0.8 mmol/L vs. 3.3 ± 1.0 mmol/L, P = 0.290). Conclusion: Our results highlight the importance of cannabidiol supplementation as a potential factor in the regulation of hyperglycemia in people with prediabetes. These findings give us an incentive to carry out further clinical research in order to determine with certainty whether the results of this research can be applied to the wider population.