Objectives: Shoulder pain is reported to be one of the most common types of musculoskeletal pain and known for causing numerous days of sick leave in the working population. Thus the aim of this study was to evaluate the effect of physical therapy on pain, mobility and functional index in order to find an appropriate treatment regimen to lessen the significant burden for the well-being of the patient and the health-care economics. Methods: A clinical trial was performed between March and June 2017 with 18 patients of the Department for physical medicine and rehabilitation of the University hospital KBC Split. The patients were from the working population and currently on sick-leave, who with the exception of two patients, were all females. Subjects were between the ages of 38 and 61. We included patients with subacromial impingement syndrome, tendinitis, subluxations, calcifications, bursitis, partial rupture of tendons and frozen shoulder and excluded patients with post traumatic shoulder complaints after fractures. Every subject underwent at least one cycle of physical therapy. Each cycle consisted of 10 sessions on 10 consecutive working days. Modalities of applied therapy were: TENS, ultrasound, cryotherapy, heat therapy, individual and group kinesiotherapy. Combinations of different modalities of therapy were used in all but one patients. Data was collected using goniometry, a translated version of the Oxford shoulder scale and an anamnestic history questionnaire. Descriptive data analysis was conducted using Excel. The research was registered with the department and accepted by the ethics committee. Results: All 18 patients completed the trial. Our results revealed that shoulder pain was most common in the years of 40-44. The most common cause of shoulder pain in our study group was calcified tendinitis. Data analysis revealed that both pain on numeric rating scale (NRS) and range of motion improved with physical therapy. Conclusion: The primary objective of our study was proven: physical therapy lessens the pain and increases the mobility in almost all patients, while secondary objectives are not statistically relevant because of the small number of patients. Further studies should be conducted.