| Abstract | Objectives: The aim of this study was to investigate plasma Matrix Gla protein (MGP) levels in patients with Crohn’s disease in comparison to control group, while additional goal was to investigate association of plasma MGP levels with disease activity and other biochemical parameters.
Materials and methods: A total of 35 patients with Crohn’s disease and 35 sex, age and BMI matched control subjects underwent anthropometric assessment which were measured by calibrated stadiometer with integrated weight scale. Venous blood samples were taken and plasma MGP levels were analyzed by Chemiluminescence immunoassay (CLIA) method using IDS-iSYS InaKtif MGP (Immunodiagnostic Systems, Frankfurt, Germany). Other biochemical parameters were determined by standard laboratory procedures.
Results: The plasma MGP levels were significantly higher in the Crohn’s disease patients in comparison to control group (641.1±141.6 vs. 532.6±98.8 pmol/L, P<0.001). Significant positive correlation was found between plasma MGP concentrations and waist circumference and hsCRP (P=0.028 and 0.024, respectively). Furthermore, MGP levels positively correlated with liver function tests. Additionally, significant negative correlation was found between MGP levels and HDL level (r=-0.242, P=0.043). There were no significant correlations between MGP levels and disease duration or fecal calprotectin level.
Conclusion: In conclusion, this study confirmed that plasma MGP levels are significantly higher in patients with Crohn’s disease in comparison to control group. Furthermore, plasma MGP levels can be related with disease activity in patients with Crohn’s disease. Further studies are needed to clarify this connection. |
| Abstract (croatian) | Cilj: Cilj ovog istraživanja bio je ispitati koncentraciju MGP-a u plazmi u bolesnika s Crohnovom bolesti u usporedbi s kontrolnom skupinom, dok je dodatni cilj bio istražiti povezanost koncentracija MGP-a u plazmi s aktivnošću bolesti i drugim biokemijskim parametrima.
Materijal i metode: Ukupno 35 bolesnika s Crohnovom bolesti i 35 zdravih ispitanika upareno prema dobi, spolu i indeksu tjelesne mase podvrgnuto je antropometrijskoj procjeni koja je mjerena kalibriranim visinomjerom s integriranom težinskom skalom. Uzorkovana je venska krv, a koncentracija MGP-a u plazmi određena je kemiluminiscencijskom imunoesej (CLIA) metodom koristeći IDS-iSYS InaKtif MGP (Immunodiagnostic Systems, Frankfurt, Njemačka). Ostali biokemijski parametri određeni su standardnim laboratorijskim postupcima.
Rezultati: Plazmatske koncentracije MGP bile su statistički značajno veće u skupini bolesnika s Crohnovom bolesti u usporedbi s kontrolnom skupinom (641,1 ± 141,6 naprema 532,6 ± 98,8 pmol / L, P <0,001). Utvrđena je značajna pozitivna korelacija između koncentracija MGP u plazmi i opsega struka i hsCRP-a (P = 0,028 i 0,024). Nadalje, koncentracija MGP-a pozitivno korelira s jetrenim enzimima. Osim toga, utvrđena je značajna negativna korelacija između koncentracije MGP-a i HDL-a (r = -0,242, P = 0,043). Nije pronađena značajna korelacija između koncentracije MGP-a i trajanja bolesti i razina fekalnog kalprotektina.
Zaključak: Ova studija je potvrdila da su koncentracije MGP-a u plazmi značajno veće u bolesnika s Crohnovom bolesti u usporedbi s kontrolnom skupinom. Nadalje, koncentracija MGP-a u plazmi može biti povezana s aktivnošću bolesti u bolesnika s Crohnovom bolesti. Potrebne su daljnje studije kako bi se pojasnila ta povezanost. |
