| Abstract | Cilj: Ispitati utjecaj konzumacije vodene otopine etanola i bijelog vina na cijeljenje akutnog infarkta miokarda u proliferacijskoj fazi (4. dan od nastanka infarkta) u odnosu na kontrolnu skupinu koja je konzumirala vodu te utvrditi izražaj α-SMA (engl. alpha smooth actin) kao markera miofibroblasta te CD68 kao markera makrofaga i moguće razlike u izražaju tih markera između kontrolne i pokusnih skupina.
Materijali i metode: Devet životinja randomizirano je u tri skupine od kojih je jedna skupina pila vodu, druga bijelo vino, a treća vodenu otopinu etanola, tijekom 28 dana. Nakon toga su podvrgnute podvezivanju prednjeg silaznog ogranka lijeve koronarne arterije, izazivajući infarkt miokarda. Nakon 72 sata, životinje su žrtvovane, srca eksplantirana te su iz njih dobiveni rezovi koji su obrađeni i imunohistokemijski obojani. Određene su tri reprezentativne peri-infarktne zone u kojima se mjerio izražaj signala već navedenih markera, α-SMA i CD68.
Rezultati: U kontekstu izražaja α-SMA, u odnosu na kontrolnu skupinu (Voda), utvrđeno je sniženje intenziteta signala za 29% (P=0,003) kod pokusne skupine Etanol, dok je za pokusnu skupinu Vino utvrđeno sniženje intenziteta signala za 18% (P=0,2). Što se tiče izražaja CD68, u odnosu na kontrolnu skupinu, kod pokusne skupine Etanol zabilježen je porast intenziteta signala od 7% (P=0,474), dok je za skupinu Vino zabilježeno sniženje intenziteta signala od 27% (P=0,119).
Zaključak: Vodena otopina etanola i vino imaju blagi utjecaj na smanjenje izražaja miofibroblasta u peri-infarktnim zonama na 4. dan cijeljenja, s tim da je statistički značajna razlika prisutna samo za vodenu otopinu etanola. Vino uzrokuje blago smanjenje izražaja makrofaga u peri-infarktnoj zoni, no bez statistički značajne razlike. |
| Abstract (english) | Aim: To examine the effect of the consumption of white wine and its aqueous ethanol solution equivalent to the proliferative phase of the infarct healing by determining the expression of α-SMA (alpha smooth actin) as a marker of myofibroblasts and CD68 as a marker of macrophages. Differences in expression are compared between water consuming controls and experimental groups.
Materials and Methods: Nine animals were randomized into three groups: water drinking controls, consumers of white wine, and consumers of 13% aqueous ethanol solution, to which they were exposed for 28 days. They were then subjected to the operative ligation of the anterior descending branch of the left coronary artery, causing myocardial infarction. 72 hours after the operation, the animals were sacrificed, the hearts were explanted, and sections were prepared and processed for immunohistochemistry. Three representative peri-infarction zones were determined and the signal expression of α-SMA and CD68 was measured and compared.
Results: When α-SMA expression was compared to the control group (water), a decrease in signal intensity of 29% (P=0.003) was found in the ethanol experimental group while for the wine group a decrease in signal intensity of 18% was found (P=0.2). Regarding the expression of CD68, compared to the control group, in the experimental ethanol group an increase in signal intensity of 7% (P=0.474) was found, while for the wine group there was a decrease in signal intensity of 27% (P=0.119).
Conclusion: Aqueous ethanol solution and white wine have a mild effect on the reduction of the myofibroblast expression in peri-infarction zones on the 4th day of healing, with a statistically significant difference present only for aqueous ethanol solution. Wine causes a mild decrease in macrophage expression in the peri-infarction zone, but without a statistically significant difference. |
