Title Volak Hexaplex trunculus kao bioindikator onečišćenja u Jadranu Title (english) Banded murex Hexaplex trunculus as bioindicator of contamination in the Adriatic Author Anita Erdelez Mentor Melita Peharda Uljević (mentor)Mentor Anamaria Štambuk (komentor)Committee member Ivana Ujević (predsjednik povjerenstva)Committee member Nevenka Mikac (član povjerenstva)Committee member Ivan Župan (član povjerenstva)Granter University of Split Defense date and country 2018-04-09, Croatia Scientific / art field, NATURAL SCIENCES Universal decimal classificationUDC ) 502/504 - Environmental science. Conservation of natural resources. Threats to the environment and protection against them Abstract Volak Hexaplex trunculus se često koristi za praćenje onečišćenja morskog okoliša organokositrovim spojevima, u prvom redu tributilkositrom (TBT). Radi se o vrsti koja razvija imposeks već pri niskim razinama onečišćenja TBT-om. U ovoj disertaciji predstavljeni su rezultati istraživanja povezanosti imposeksa kod volka H. trunculus i onečišćenja TBT-om provedenih na području srednjeg dijela hrvatskog Jadrana na 7 postaja tijekom 2014. godine i 12 postaja tijekom 2015. godine. Kod svih istraživanih populacija zabilježena je pojava imposeksa, a mjerenjem masenog udjela TBT-a u tkivu volka H. trunculus i sedimentu potvrđena je i prisutnost onečišćenja TBT-om. Istraživanjem provedenim 2014. godine nije potvrđena povezanost onečišćenja TBT-om s aktivnošću odabranih biomarkera (katalaza, glutation S- transferaza, glutation reduktaza, malondialdehidi, karbonili, acetilkolinesteraza), iako je uočena povećana aktivnost glutation reduktaze i glutation S-transferaze kod volaka s jače onečišćenih postaja. Istraživanje učinkovitosti zabrane korištenja TBT-a, koja je u Republici Hrvatskoj na snazi od 2006. godine, provedeno je 2015. godine na istim postajama na kojima je istraživanje imposeksa provedeno i 2005. godine, kategoriziranim prema razini brodske aktivnosti (nautičke marine, lučice, uvale koje se koriste samo sezonski, referentne postaje koje se rijetko koriste). Rezultati istraživanja sugeriraju da je u desetogodišnjem razdoblju došlo do smanjenja razine onečišćenja TBT-om. Ipak, smanjenje nije rezultiralo oporavkom istraživanih populacija budući da je kod svih utvrđen visoki udio imposeksa kao i povećanje udjela sterilnih ženki. U svrhu potencijalnog korištenja vrste H. trunculus kao bioindikatora u ocjeni ekološkog stanja priobalnih voda u okviru Direktive o vodama, a s obzirom na onečišćenje voda TBT-om i posljedičnim razvojem imposeksa, predložene su granične vrijednosti omjera ekološke kakvoće (OEK) . Istraživanjem stabilnih izotopa dušika (Ł15Nair) i ugljika (Ł13CVPDV) u tkivu volka H. trunculus nije potvrđena povezanost sadržaja stabilnih izotopa sa stupnjem onečišćenosti istraživanih postaja TBT-om.
Abstract (english) Banded murex Hexaplex trunculus is used in the marine environment monitoring of organotin pollution, as this species develops imposex at very low tribytiltin (TBT) concentration. In this thesis relationship between imposex in H. trunculus and TBT pollution was investigated along the central Croatian Adriatic coast at 7 sites in 2014 and 12 sites in 2015. Imposex was recorded at all investigated sites on which also TBT pollution was demonstrated, both in tissue of H. trunculus and in sediment. Investigations performed in year 2014 showed no connection between degree of TBT pollution and activity of specific biomarkers (catalase, glutathione S-transferase, glutathione reductase, malondialdehydes, carbonyls, acetylcholinesterase) in gastropod, although the increased activity of glutathione reductase and glutathione S-transferase in banded murex from the most polluted sites was observed. Investigation on effectiveness of the ban of organotin compounds in antifouling coatings, which is in force in the Republic of Croatia since 2006, was performed in 2015 at the same sites previously investigated in 2005 which were categorized according to intensity of boating activities to nautical marinas, village harbours, sheltered bays and reference sites. Obtained results suggested that TBT pollution has decreased in this ten years period. However, this decrease was not sufficient for the recovery of H. trunculus populations, since in 2015 all populations showed high degree of imposex and even higher number of sterile females than in 2005. The Ecological Quality Ratio (EQR) values for the potential use of imposex in H. trunculus as bioindicator in assessment of the ecological status of coastal waters regarding TBT pollution in the frame of Water Framework Directive (WFD) are proposed. Relationship between carbon (Ł13CVPDV) and nitrogen (Ł15Nair) isotopes content in H. trunculus and degree of TBT pollution was not established.
Keywords
biomarkeri
Hexaplex trunculus
imposeks
ocjena ekološkog stanja priobalnih voda
onečišćenje TBT-om
srednji Jadran
stabilni izotopi
Keywords (english)
assessment of the ecological status of coastal waters
biomarkers
central Adriatic
Hexaplex trunculus
imposex
stable isotopes
TBT pollution
Language croatian URN:NBN urn:nbn:hr:226:202631 Study programme Title: Applied Marine Sciences Type of resource Text Extent XI, 112 str ; 30 cm File origin Born digital Access conditions Open access Terms of use Created on 2018-05-14 06:32:32
