Title Dijagnostika infekcija uzrokovanih bakterijama Ureaplasma urealyticum i Mycoplasma hominis
Title (english) Diagnosis of infections caused by Ureaplasma urealyticum and Mycoplasma hominis
Author Tina Kavelj
Mentor Anita Novak (mentor)
Committee member Sendi Kuret (predsjednik povjerenstva)
Committee member Vanja Kaliterna (član povjerenstva)
Committee member Anita Novak (član povjerenstva)
Granter University of Split (University Department of Health Studies) (Chair of Medical Laboratory Diagnostics) Split
Defense date and country 2022-07-04, Croatia
Scientific / art field, discipline and subdiscipline BIOMEDICINE AND HEALTHCARE Clinical Medical Sciences Medical Microbiology
Abstract Cilj rada: Svrha istraživanja je bila odrediti učestalost infekcija uzrokovanih M. hominis i U. urealyticum u KBC-u Split od 1.1.2020 do 31.12.2020 te odrediti osjetljivost navedenih izolata na antibiotike u istom periodu.
Materijali i metode: Istraživanje je obuhvatilo podatke o pacijentima s dokazanom M. hominis ili U. urealyticum u navedenom razdoblju. Podatci su prikupljeni u Kliničkom zavodu za mikrobiologiju i parazitologiju, Kliničkog bolničkog centra (KBC) Split. Kao dijagnostička metoda koristio se komercijalni kultivacijski set MYCOFAST RevolutioN, podijeljen u dvije faze testa. Probirna faza ispituje prisutnost (pozitivni rezultat) ili odsutnost (negativni rezultat) mikoplazmi i ureaplazmi u uzorku. Druga faza se provodi na uzorcima kojima je dokazana mikoplazma ili ureaplazma u probirnom testu, a njena svrha je identifikacija i kvantifikacija izolirane bakterije te ispitivanje njene osjetljivosti na antibiotike. Princip obje faze testa je sličan, jažice setova ispunjene su tekućim medijem koji sadrži hranjive tvari potrebne za rast obje bakterije, specifične antibiotike kojima je moguće identificirati ili odrediti osjetljivost bakterija te indikator koji vizualizira porast bakterija u jažici.
Rezultati: Klinički zavod za mikrobiologiju i parazitologiju u Splitu zaprimio je 638 uzoraka suspektnih na infekciju ureaplazmom i mikoplazmom. Od toga je 590 uzoraka bilo negativno, a u 7% (45 uzoraka) je dokazana U. urealyticum te 0,5% (3 uzorka) M. hominis. Ureaplazme najčešće izazivaju infekcije genitalnog sustava i to u žena u generativnoj dobi. Većina testiranih izolata bila je osjetljiva na doksicikin (94,4 %), eritromicin (82,9 %), moksifloksacin (80,5 %), levofloksacin (77,8 %) i tigeciklin (76 %), a rezistentna na klindamicin (95,8 %).
Zbog malog broja dokazanih mikoplazmi, uzorak je nereprezentativan i nije rađena statistička analiza.
Abstract (english) Objectives: The study aimed to determine the incidence of infections caused by M. hominis and U. urealyticum at the University Hospital of Split from 1.1.2020 to 31.12.2020 and to determine the susceptibility of these isolates to antibiotics in the same period.
Materials and methods: The study was carried out on data on patients with isolated M. hominis or U. urealyticum during this period. The data were collected from the Department of Microbiology and Parasitology, University Hospital of Split (UHS). As a diagnostic method, the commercial cultivation set MYCOFAST RevolutioN was used, divided into two phases of the test. The screening phase examines the presence (positive result) or absence (negative result) of mycoplasma and ureaplasma in the sample. The second phase is carried out on samples that have been proven mycoplasma or ureaplasma in the screening test, and its purpose is to identify and quantify the isolated bacterium and to examine its susceptibility to antibiotics. The principle of both phases of the test is similar, the wells of the sets are filled with a liquid medium containing the nutrients necessary for the growth of both bacteria, specific antibiotics with which it is possible to identify or determine the sensitivity of bacteria, and an indicator that visualizes the increase of bacteria in the well.
Results: Department of Microbiology and Parasitology UHS received 638 samples suspected of infection with ureaplasma and mycoplasma. Of these, 590 samples were negative, and in 7% (45 samples) U. urealyticum and 0.5% (3 samples) M. hominis were proven. Ureaplasma was predominantly isolated from the genital system in women of generative age. The majority of tested strains were susceptible to doxycycline (94,4 %), erythromycin (82,9 %), moxifloxacin (80,5 %), levofloxacin (77,8 %) and tigecycline (76 %); and resistant to clindamycin (95,8 %).
However, the sample size for mycoplasma was too small for statistical analysis.
Keywords
dijagnostika U. urealyticum i M. hominis
kultivacija
infekcije mokraćno-spolnog sustava
Keywords (english)
diagnostics of U. urealyticum and M. hominis
cultivation
urinary-sexual system infections
Language croatian
URN:NBN urn:nbn:hr:176:303757
Study programme Title: Medical Laboratory Diagnostics (university/undergraduate) Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike (sveučilišni prvostupnik/ prvostupnica (baccalaureus/ baccalaurea) medicinske laboratorijske dijagnostike)
Type of resource Text
File origin Born digital
Access conditions Open access
Terms of use
Created on 2022-07-06 08:13:09