Title Povezanost migrene i alela HLA DRB1* Title (english) ASSOCIATION OF MIGRAINE AND HLA DRB1* ALLELES Author Tina Bitunjac Mentor Esma Čečuk-Jeličić (mentor)Committee member Daniela Šupe-Domić (predsjednik povjerenstva)Committee member Dejana Bogdanić (član povjerenstva)Committee member Esma Čečuk-Jeličić (član povjerenstva)Granter University of Split Defense date and country 2022-07-28, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Sustav HLA najsloženiji je genetski sustav kod čovjeka. Između ostalog, osobitosti ovog
sustava su izraziti polimorfizam, kao i povezanost ovog sustava s bolestima, što je od
izrazite važnosti u dijagnostici autoimunih bolesti kao i neuroloških oboljenja. Migrena je
ponavljajuća, umjereno jaka do vrlo jaka glavobolja u trajanju između 4 i 72 sata u slučaju
kada se ne liječi. Postoji nekoliko čimbenika rizika povezanih s razvojem migrene. U
nepromjenjive čimbenike spadaju genetika, dob i spol.
Cilj je ovog rada odrediti zastupljenost alela sustava HLA u pacijenata kojima je
dijagnosticirana migrena i kod pacijenata koji pate od jakih glavobolja na području Splitskodalmatinske županije kao i prikazati zastupljenost pojedinih alela lokusa HLA - DRB1*.
Kod ispitanika s migrenom i jakim glavoboljama koji su imali HLA-DRB1*16,
zastupljenost spolova pokazuje da je znatno veći postotak oboljelih kod žena (68.96%) u
odnosu na muškarce (31.04%). Najveći broj ispitanika je bio u dobnoj skupini od 46 do 65
godina.
Antigeni i aleli sustava HLA za potrebe ove studije određeni su serološkim i
molekularnim metodama. Antigeni sustava HLA određuju se testom
mikrolimfocitotoksičnosti (MLCT), dok su za molekularno određivanje alela lokusa sustava
HLA korištene metode PCR-SSO i PCR-SSP.
Određivanjem alela lokusa HLA-DRB1 u skupini ispitanika s migrenom, uočeno je 14
različitih alela, od kojih najveću zastupljenost imaju HLA-DRB1*16 (19,59%) te HLADRB1*11,-13,-15 s učestalosti od 10,14%. U usporedbi sa kontrolnom skupinom, utvrđeno
je da je učestalost alela HLA-DRB1*16 (19,59% vs 9,60%, p=0,0063) i HLA-DRB1*10
(2,7% vs 0%, p=0,0327) statističko značajno povećana u skupini pacijenata koji boluju od
migrene ili jake glavobolje.
Budući da su našim ispitanicima u dijagnostičke svrhe određivani i aleli lokusa HLA
razreda I (HLA-A, HLA-B) prilikom analize uočena je statistički značajna povezanost alela
HLA-B*57 kod pacijenata s migrenom i jakim glavoboljama u odnosu na kontrolnu skupinu
zdravih ispitanika.
Da bismo sa sigurnošću govorili o povezanosti migrene i jakih glavobolja i alela HLADRB1*16 i ostalih alela lokusa sustava HLA (HLA-A, HLA-B) svakako je potrebno
proširiti studiju na veći broj ispitanika.
Abstract (english) The HLA system is the most complex genetic system in humans. Among other things, the
peculiarities of this system are its pronounced polymorphism, as well as the connection of this
system with diseases, which is of great importance in the diagnosis of autoimmune diseases as
well as neurological diseases. Migraine is a recurrent, moderately severe to very severe
headache lasting between 4 and 72 hours if left untreated. There are several risk factors
associated with developing migraines. Immutable factors include genetics, age and gender.
The objective of this work was to determine the representation of alleles of the HLA system in
patients suffering from migraines and severe headaches in the Split-Dalmatia County, as well
as to show the representation of individual alleles of the HLA locus - DRB1*.
In subjects with migraine and severe headaches who had HLA-DRB1*16, the representation of
genders shows that the percentage of patients is significantly higher in women (68.96%)
compared to men (31.04%). The largest number of respondents was in the age group of 46 to
65 years.
Antigens and alleles of the HLA system for the purposes of this study were determined by
serological and molecular methods. Antigens of the HLA system are determined by the
microlymphocytotoxicity test (MLCT), while the PCR-SSO and PCR-SSP were used for the
molecular determination of alleles of the HLA system locus. .
By determining the alleles of the HLA-DRB1 locus in the group of subjects with migraine, 14
different alleles were observed, of which the highest prevalence is HLA-DRB1*16 (19.59%)
and HLA-DRB1*11,-13,-15 with a frequency of 10 , 14%. Compared to the control group, the
frequency of alleles HLA-DRB1*16 (19.59% vs 9.60%, p=0.0063) and HLA-DRB1*10 (2.7%
vs 0%, p =0.0327) statistically significantly increased in the group of patients with migraine or
severe headache.
Since alleles of the HLA class I locus (HLA-A, HLA-B) were also determined for diagnostic
purposes in our subjects, the analysis revealed a statistically significant association of the HLAB*57 allele in patients with migraine and severe headaches compared to the healthy control
group examinee.
In order to talk with certainty about the connection between migraine and severe headaches and
the HLA-DRB1*16 allele and other alleles of the HLA system locus (HLA-A, HLA-B), it is
certainly necessary to expand the study to a larger number of subjects.
Keywords
HLA
alel
migrena
PCR-SSP
PCR-SSO
MLCT
Keywords (english)
HLA
allele
migraine
PCR-SSP
PCR-SSO
MLCT
Language croatian URN:NBN urn:nbn:hr:176:823097 Study programme Title: Medical Laboratory Diagnostics (university/undergraduate) Type of resource Text File origin Born digital Access conditions Open access Terms of use Created on 2022-07-28 08:13:41
