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undergraduate thesis
Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi
Split: University of Split, 2019. urn:nbn:hr:176:333405
Urlić, Marta
University of Split
University Department of Health Studies

Institutional repository: Repository of the University Department for Health Studies, University of Split

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Urlić, M. (2019). Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, Marta. "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi." Undergraduate thesis, University of Split, 2019. https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, Marta. "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi." Undergraduate thesis, University of Split, 2019. https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić, M. (2019). 'Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi', Undergraduate thesis, University of Split, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:176:333405

Urlić M. Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi [Undergraduate thesis]. Split: University of Split; 2019 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:176:333405

M. Urlić, "Komercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi", Undergraduate thesis, University of Split, Split, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:176:333405

Metadata
TitleKomercijalni STR sustavi: vrijednost i doprinos u forenzičnoj DNA analizi
Title (english)COMMERCIAL STR KITS: VALUE AND CONTRIBUTION IN A FORENSIC DNA ANALYSIS
AuthorMarta Urlić
MentorDavorka Sutlović (mentor)
Committee memberDavorka Sutlović (predsjednik povjerenstva)
Committee memberIrena Drmić Hofman (član povjerenstva)
Committee memberDaniela Šupe-Domić (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2019-09-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
Ciljevi rada: Prikazati identifikacijske STR setove te usporediti njihovu vrijednost i značajnost u komercijalne svrhe. Metode: Opisano je pet različitih STR sustava koji se koriste u forenzičnoj DNA analizi u različite svrhe. Sadržaj svakog seta omogućuje izvođenje PCR metode pri čemu se umnaža DNA iz raznih bioloških uzoraka te se amplificirani produkti razdvajaju pa detektiraju i naposlijetku analiziraju. Rezultati: MiniFiler set reagensa najbolje se koristi u analizi malih količina DNA, asadrži 8 različitih lokusa za umnažanje DNA. SGM Plus identifikacijski kit sadrži 2 lokusa više, što doprinosi većoj osjetljivosti analize. Identifiler Plus i Power Plex koriste čak 15 STR lokusa te jedan spolno determinirajući marker – Amelogenin, što dodatno doprinosi njihovoj iznimnoj snazi diskriminacije. Zaključci: Otkriće komercijalnih STR-sustava označilo je prekretnicu u forenzičnoj DNA analizi, a uvođenje sve više lokusa u pojedine setove rezultiralo je povećanjem njihove snage diskriminacije i na taj način svelo pojavnost pogrešaka na minimum.
Abstract (english)
Aim: To present STR kits for forensic identification and compareits value and relevance for commercial purposes. Methods: Five different STR kits in use for forensic DNA analyis have been described. The content of the each kit enables the performance of PCR amplification of a DNA from different sources. The amplified products were separated and set up for detection and interpretation. Results: The MiniFiler kit was the best used, especially in analysis of the small amonuts of degraded DNA, and amplified 8 different loci. Two more loci are included in the SGM Plus identification kit which results in a much higher sensitivity rate. The Identifiler Plus and the PowerPlex 16 amplification kits amplify 15 different STR loci and one gender-determining marker – Amelogenin, which strongly contributes to a greater power of discrimination. Conclusions: Discovery of the commercial STR-kits marked a turning point in the forensic DNA analysis, and by including more and more loci in each set has enhanced the power of discrimination, which led to a error rate reduction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:333405
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-06-23 09:03:29