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undergraduate thesis
Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora
Split: University of Split, 2014. urn:nbn:hr:176:585476
Knežević, Mirjana
University of Split
University Department of Health Studies

Institutional repository: Repository of the University Department for Health Studies, University of Split

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Knežević, M. (2014). Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora (Undergraduate thesis). Split: University of Split. Retrieved from https://urn.nsk.hr/urn:nbn:hr:176:585476

Knežević, Mirjana. "Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora." Undergraduate thesis, University of Split, 2014. https://urn.nsk.hr/urn:nbn:hr:176:585476

Knežević, Mirjana. "Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora." Undergraduate thesis, University of Split, 2014. https://urn.nsk.hr/urn:nbn:hr:176:585476

Knežević, M. (2014). 'Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora', Undergraduate thesis, University of Split, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:176:585476

Knežević M. Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora [Undergraduate thesis]. Split: University of Split; 2014 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:176:585476

M. Knežević, "Izražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora", Undergraduate thesis, University of Split, Split, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:176:585476

Metadata
TitleIzražaj biljega CD34 i CD77 na plazma membrani srčanih stanica hiperkalcijemičnih štakora
Title (english)Expression of Markers CD34 and CD77 on the Plasma Membrane of Hypercalcemic Rats Heart Cells
AuthorMirjana Knežević
MentorVedrana Čikeš Čulić (mentor)
Committee memberAnita Markotić (predsjednik povjerenstva)
Committee memberMirela Zec (član povjerenstva)
Committee memberVedrana Čikeš Čulić (član povjerenstva)
GranterUniversity of Split
(University Department of Health Studies)
Split
Defense date and country2014-07-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract
CILJ: Odrediti izražaj CD77 molekule na CD34+ stanicama bubrega štakora koji su dobivali 14 dana prirodnu vodu obogaćenu kalcijevim kloridom (15%-tna koncentracija elementarnog kalcija ) u usporedbi s izražajem CD77 na CD34+ stanicama bubrega štakora koji su dobivali prirodnu vodu. METODE: Svi štakori su uzgojeni pod kontroliranim uvjetima, s tim da su bili podijeljeni u dvije skupine: ispitivana skupina koja je bila tretirana vodom obogaćenom kalcijem i kontrolna skupina koja je dobivala prirodnu vodu. Nakon 14 dana određena je koncentracija ionskog kalcija u plazmi, mjerena je ion selektivnom elektrodom na Blood Analyzern Rapid Lab Model 348. Nakon žrtvovanja hiperkalcijemičnih i kontrolnih štakora, bubrezi su izvađeni iz svih životinja. Izolirane stanice bubrega inkubirane su sa dva protutijela za dvostruko bojanje stanica: protutijelo na CD34 konjugirano s PC5 i CD77 konjugirano s FITC-om, te analizirane protočnom citometrijom. REZULTATI: Postotak ionskog kalcija bio je 48±0.30 % u hiperkalcijemičnim naspram 44±0.96 % u kontrolnim štakorima (P=0.013). U bubregu hiperkalcijemičnih štakora postotak CD34+CD77- stanica je niži u odnosu na kontrolnu skupinu(P=0.043). ZAKLJUČAK ISTRAŽIVANJA: U ovom istraživanju određen je izražaj biljega CD77 na CD34 pozitivnim endotelnim stanicama u suspenzijama stanica tkiva bubrega. CD34+CD77- stanice bubrega u ovom istraživanju pokazali su visoku osjetljivost na povišen unos kalcija. Hiperkalcijemija je rezultirala smanjenjem udjela endotelnih CD34+CD77- stanica u bubregu.
Abstract (english)
OBJECTIVE: To determine the expression of CD77 molecule on CD34+kidney cells of rats that received 14 days natural water enriched with calcium chloride (15% concentration of elemental calcium) in comparison to the expression of CD77 on CD34+ kidney cells of rats that received natural water. METHODS: All rats were grown under controlled conditions, divided into two groups: a test group, which was treated with water enriched with calcium and a control group, receiving the natural water. After 14 days, the concentration of ionic calcium in the plasma was measured by ion selective electrode on Blood Analyzern Rapid Lab Model 348. After sacrificing, hypercalcemic and control rat kidneys were removed from all animals. Isolated kidney cells were incubated with the two antibodies for double staining: anti-CD34 conjugated with PC5 and anti-CD77 conjugated with FITC, and analyzed by flow cytometry. RESULTS: The percentage of ionic calcium was 48% ± 0:30 in hypercalcemic rats comparing with 44 ± 0.96% in the control rats (P = 0.013). In the kidney of hypercalcemic rats percentage of CD34+CD77- cells was lower than in the control group (P=0.043). CONCLUSION: The present study determined the expression of the marker CD77 on CD34 positive endothelial cells in cell suspensions of kidney tissue. CD34+CD77- kidney cells in this study showed high sensitivity to increased calcium intake. Hypercalcemia resulted in a decrease of endothelial CD34+CD77-cells in the kidney.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:176:585476
Study programmeTitle: Medical Laboratory Diagnostics (university/undergraduate)
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika (sveučilišni prvostupnik/prvostupnica (baccalaureus/baccalaurea) medicinska laboratorijska dijagnostika)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2020-06-29 13:49:57