Title Dijagnostika hepatitisa C Title (english) DIAGNOSTIC OF HEPATITIS C Author Lea Marušić Mentor Vanja Kaliterna (mentor)Committee member Esma Čečuk-Jeličić (predsjednik povjerenstva)Committee member Anita Novak (član povjerenstva)Committee member Vanja Kaliterna (član povjerenstva)Granter University of Split Defense date and country 2020-07-30, Croatia Scientific / art field, BIOMEDICINE AND HEALTHCARE Abstract Cilj: Cilj rada bio je opisati korištenu metodu dijagnostike HCV-a, interpretirati dobivene rezultate, utvrditi postotak pozitivnih HCV rezultata u NZJZ SDŽ i postoji li razlika u postotku pozitivnih rezultata na HCV između dvije ispitivane skupine (anonimno testirani i pacijenti).
Metode: Metoda detekcije koju koristi NZJZ SDŽ je ELFA (engl. enzyme linked fluorescent assay). VIDAS® Anti-HCV je automatski kvalitativni test koji se koristi za detekciju anti-HCV iz ljudskog seruma ili plazme. Princip testa kombinira dvokorakno enzimsko imunološko ispitivanje s konačnim flourescencijskim očitavanjem čija se fluorescencija mjeri na 450 nm. Analiza rezultata vrši se automatski pomoću kompjutera. Rezultati se na nalazima izražavaju kvalitativno. Vrijednost testa jednaka je omjeru relativne fluorescencijeske vrijednosti pacijenta i one standarda. Ako je vrijednost testa ≥ 1,00, smatra se da je pacijent pozitivan na anti-HCV protutijela, a ako je < 1,00, rezultat se interpretira kao negativan.
Rezultati: U 2019. godini na Nastavnom zavodu za javno zdravstvo Splitsko-dalmatinske županije ukupno je testirano 1577 osoba, od čega je 56 osoba (3,6 %) bilo pozitivno na anti-HCV. Od ukupnog broja testiranih, njih 416 (26,4 %) testirano je anonimno i upućeno iz Službe za epidemiologiju. Nije utvrđena statistički značajna razlika u učestalosti pozitivnih nalaza na hepatitis C u dvije ispitivane skupine, pacijenti i anonimno testirani (p = 0.491568).
Zaključak: U 2019. godini na Nastavnom zavodu za javno zdravstvo Splitsko-dalmatinske županije, korištenjem VIDAS® Anti-HCV automatskog kvalitativnog testa za detekciju IgG protutijela virusa hepatitisa C iz ljudskog seruma ili plazme pomoću ELFA metode, ukupno je testirano 1577 osoba, od čega je 56 osoba (3,6 %) bilo pozitivno na anti-HCV, a nije utvrđena statistički značajna razlika u učestalosti pozitivnih nalaza na hepatitis C u dvije ispitivane skupine (pacijenti i anonimno testirani).
Abstract (english) Objective: The aim of the study was to describe the used method of HCV diagnosis, to interpret the results, to determine the percentage of positive HCV results in NZJZ SDŽ and whether there is a difference in the percentage of positive HCV results between two examined groups (anonymously tested and patients).
Methods: The detection method used by NZJZ SDŽ is ELFA (enzyme linked fluorescent assay). VIDAS® Anti-HCV is an automatic qualitative test used to detect anti-HCV from human serum or plasma. The principle of the test combines a two-step enzyme-linked immunosorbent assay with a final fluorescence reading measured at 450 nm. The analysis of the results is done automatically by computer. The results are expressed qualitatively. The test value is equal to the ratio of the relative fluorescence value of the patient to that of the standard. If the test value is ≥ 1.00, the patient is considered positive for anti-HCV antibodies, and if < 1.00, the result is interpreted as negative.
Results: In 2019, a total of 1577 people were tested at the Teaching Institute for Public Health of Split-Dalmatia County, of which 56 (3.6 %) were positive for anti-HCV. Out of the total number, 416 of them (26.4 %) were tested anonymously and were sent from the Epidemiology Service. Statistically significant difference was not found in the frequency of positive hepatitis C findings in the two study groups, patients and those tested anonymously (p = 0.491568).
Conclusion: In 2019, at the Teaching Institute for Public Health of Split-Dalmatia County, using the VIDAS® Anti-HCV automatic qualitative test for the detection of IgG antibodies to hepatitis C virus from human serum or plasma using ELFA method, a total of 1577 people were tested, of which 56 (3.6 %) were positive for anti-HCV, and statistically significant difference was not found in the frequency of positive hepatitis C findings in the two study groups (patients and anonymously tested).
Keywords
dijagnostika hepatitisa C
flavivirusi
medicinsko laboratorijska dijagnostika (ključne riječi unio urednik)
Keywords (english)
hepatitis C diagnosis
flaviviruses
medical laboratory diagnostics (ključne riječi unio urednik)
Language croatian URN:NBN urn:nbn:hr:176:997772 Study programme Title: Medical Laboratory Diagnostics (university/undergraduate) Type of resource Text File origin Born digital Access conditions Access restricted to students and staff of home institution Terms of use Created on 2020-08-04 13:10:26
