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master's thesis
Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:104089
Srzić, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Srzić, M. (2012). Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:104089

Srzić, Marina. "Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:104089

Srzić, Marina. "Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:104089

Srzić, M. (2012). 'Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:104089

Srzić M. Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 August 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:104089

M. Srzić, "Djelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:104089

Metadata
TitleDjelovanje 17α-etinilestradiola i dietilstilbestrola na Channel Catfish Ovary (CCO) stanice prilagođene na rast u mediju bez seruma
Title (english)Effects of 17α-ethynylestradiol and diethylstilbestrol on Channel Catfish Ovary (CCO) cells adapted to growth in serum-free medium
AuthorMarina Srzić
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2012-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Za ispitivanje učinaka endokrinih modulatora koji su prisutni u okolišu sve više se rabe in vitro testovi toksičnosti. Pri ispitivanju in vitro citotoksičnosti, stanice se najčešće uzgajaju u mediju kojem je dodan serum životinjskog podrijetla koji je bogat faktorima rasta i proteinima, no u novijim istraživanjima provode se testovi u mediju bez seruma. Cilj ovog rada bio je ispitati učinak 17α-etinilestradiola (EE2) i dietilstilbestrola (DES) na CCO stanice koje su prilagođene na rast u mediju bez seruma (CCO-SF). Stanice su tretirane EE2 i DESom u koncentracijama od 0,1 do 10 µg/mL u UC mediju bez seruma. Tijekom 72 sata od tretmana, vijabilnost CCO-SF stanica određena je WST-1 metodom. Na temelju dobivenih rezultata i usporedbe EC50vrijednosti s vrijednostima za CCO stanice uzgojenim u mediju sa serumom pokazano je da je citotoksični učinak EE2 i DES-aizraženiji u mediju sa serumom.
Abstract (english)
The in vitro tests are frequently used to determine the impact of endocrine modulators present in environment. During the in vitro testing of citotoxicity, the cells are grown in the media with added serum of animal origin, which contains growth factors and proteins, but recent research are conducted in serum-free conditions. The aim of this study was to investigate the effect of 17α-ethynylestradiol (EE2) and diethylstilbestrol (DES) on CCO cells cultivated in comercial serum-free medium. Cells were treated with EE2 and DES and EE2 in concentration between 0,1 and 10 µg/ml in UC medium without serum. During 72 hours of treatment, the viability of CCO cells has been determined by WST-1 method. The obtained results and comparison with those gained with CCO cells grown in compleated medium with serum showed that cytotoxic effects of EE2 and DES is more pronounced in medium with serum added.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:104089
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-09 11:02:03