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master's thesis
Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:308730
Herceg, Luka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Herceg, L. (2012). Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:308730

Herceg, Luka. "Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:308730

Herceg, Luka. "Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:308730

Herceg, L. (2012). 'Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:308730

Herceg L. Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:308730

L. Herceg, "Genotoksični učinak klenbuterola u in vitro i in vivo uvjetima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:308730

Metadata
TitleGenotoksični učinak klenbuterola u in vitro i in vivo uvjetima
Title (english)Genotoxicity effects of clenbuterol in vitro and in vivo
AuthorLuka Herceg
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Klenbuterol je simpatotimetički amin i derivat katekolamina epinefrina i norepinefrina te pripada skupini lijekova zvanih β-agonisti. Uporaba β-agonista u anaboličke svrhe rezultira kumulacijom farmakološki aktivnih ostataka u mesu tretiranih životinja te su zabilježeni brojni slučajevi akutnih otrovanja kod ljudi nakon konzumacije jetre tretiranih životinja. U ovom radu istraživan je citotoksični i prooksidativni učinak klenbuterola na humanim stanicama u kulturi, genotoksični učinak na ljudske limfocite in vitro, te genotoksični učinak na stanice jetre i krvi zamorčadi in vivo. Istraživanje citotoksičnosti i prooksidativnog učinka na humanim stanicama u kulturi pokazalo je potencijalno mitogeni učinak pri dozi 10 puta većoj od NOAEL vrijednosti, te prooksidativni učinak pri istoj koncentraciji. Pri koncentraciji od 25 μg/L klenbuterol izaziva lomove u molekuli DNA ljudskih limfocita tretiranih u uvjetima in vitro. U in vivo uvjetima, klenbuterol je bio izrazito citotoksičan na stanice jetre zamorčadi, dok se u preživjelim stanicama nije pokazao njegov genotoksični karakter. U limfocitima zamorčadi klenbuterol se pokazao kao genotoksičan spoj u ovisnosti o koncentraciji.
Abstract (english)
Clenbuterol is a symphatomimetic amine derivative of catecholamines epinephrine and norepinephrine and belongs to a class of drugs called β-agonists. The use of β-agonists in anabolic purposes resulted in acumulation of pharmacologically active residues in meat of treated animals, and there were many recorded cases of acute poisoning in humans after consumption of the liver of treated animals. In this thesis, we investigated the cytotoxic and prooxidant effects of clenbuterol in human cells in culture, genotoxic effects on human lymphocytes in vitro, and evaluated the effects on the cells of the liver and blood of guinea pigs in vivo. Research of cytotoxic and prooxidative effect on human cells in culture showed potentially mitogenic effect at a dose 10 times higher than the NOAEL values and prooxidant effect at the same concentration. At a concentration of 25 μg/L, there were DNA aberations in human lymphocytes in vitro. In vivo, clenbuterol was extremely cytotoxic to liver cells of guinea pigs, while in the surviving cellsno genotoxicity was observed . In lymphocytes of guinea pigs clenbuterol has proven to be a genotoxic compound dependent on the concentration applied.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:308730
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-10 09:11:56