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undergraduate thesis
Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:144174
Cepanec, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Fermentation and Yeast Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Cepanec, M. (2012). Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:144174

Cepanec, Maja. "Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:144174

Cepanec, Maja. "Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:144174

Cepanec, M. (2012). 'Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:144174

Cepanec M. Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:144174

M. Cepanec, "Usporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:144174

Metadata
TitleUsporedba rasta, sadržaja SAMe i tolerancije na etanol divljeg soja P. stipitis i mutanta M12 NisR
Title (english)Comparison of growth, content of SAM and ethanol tolerance of wild strain of P. stipitis and nistatin - resistant mutant M12
AuthorMaja Cepanec
MentorDamir Stanzer (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Fermentation and Yeast Technology)
Zagreb
Defense date and country2012-06-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kvasac Pichia stipitis je zanimljiv zbog sposobnosti unutarstaničnog akumuliranja (S)-adenozil-L-metionina (SAMe). Mutanti dobiveni ultraljubičastim svjetlom i rezistentni na nistatin imaju promijenjen sastav sterola u staničnoj membrani, odnosno mogu nakupljati SAMe koji se ne iskoristi u biosintezi ergosterola. Cilj ovog rada bio je usporediti karakteristike (potrošnja glukoze, prirast biomase, sadržaj SAMe u biomasi kvasca, tolerancija na etanol) divljeg soja P. stipitis i mutiranog soja M12 (UV mutant NisR) tijekom uzgoja na tresilici u kemijski definiranim podlogama. Uspoređujući ova dva soja primijećen je znatno brži rast odnosno potrošnja glukoze divljeg soja kvasca P. stipitis u odnosu na mutirani soj M12. Postignut je znatno veći sadržaj SAMe u biomasi mutiranog soja M12 uzgojenog u mediju uz dodatak 1,5 g/L D,L-metionina u usporedbi s divljim sojem P. stipitis, dok je u mediju bez dodatka D,L-metionina uočena neznatna razlika u količini SAMe prisutnog u biomasi ispitivanih sojeva kvasca. Utvrđena je i niža tolerancija na etanol mutiranog soja M12 u usporedbi s divljim sojem P. stipitis.
Abstract (english)
Yeast Pichia stipitis is interesting because of its ability to intracellular concentrations of (S)-adenosyl-L-methionine. Mutants obtained by ultraviolet radiation and resistant to nystatin have altered sterol composition within cellular membrane and can accumulate SAM that is not utilaze in the ergosterol biosynthesis. The aim of this work was to compare the characteristics (consumption of glucose, biomass growth, content of SAM in yeast biomass, tolerance to ethanol) of P.stipitis wild strain and mutant M12 (obtained by UV and resistant to nystatin) during cultivation in a chemically defined media in a shaker. Comparing these two strains, much more rapid growth and consumption of glucose were observed in wild yeast strain P. stipitis than in mutant M12. The amount of SAM in the biomass of mutant M12 grown in medium with the addition of 1.5 g/L D,L-methionine was significantly higher compared to the wild strain P. stipitis, while in the medium without the addition of D, L-methionine only slight differences in the amount of SAM present in the tested strains of yeast were revealed. A lower tolerance to ethanol was determined in the mutant M12 compared to the wild strain of P. stipitis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:144174
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
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Created on2017-05-12 10:52:52