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undergraduate thesis
Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:798207
Nimac, Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Nimac, M. (2012). Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:798207

Nimac, Marija. "Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:798207

Nimac, Marija. "Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:798207

Nimac, M. (2012). 'Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:798207

Nimac M. Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:798207

M. Nimac, "Određivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:798207

Metadata
TitleOdređivanje oblika mutacije prema dobivenim rezultatima križanja vinske mušice Drosophila melanogaster
Title (english)Determining the shape of the mutation from results obtainedby crossing fruit fly Drosophila melanogaster
AuthorMarija Nimac
MentorLidija Šver (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-09-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj rada je bio, na osnovu broja potomaka križanja kroz nekoliko generacija, odrediti da li je mutacija bijele boje očiju u vinske mušice Drosophila melanogaster mutacija nasljednog svojstva vezana uz spol ili dvostruka autosomalna mutacija. Za potrebe križanja, u staklenke s prethodno pripremljenom podlogama nasađivali smo po 5 mužjaka i 5 djevičanskih ženki. Brojanjem potomaka križanja divljeg tipa mušica i bijelih mušica kroz nekoliko generacija utvrđena je dvostruka autosomalna mutacija jer su postignuta 4 različita fenotipa smeđe, crvene, grimizne i bijele oči. Crvena boja očiju vinske mušice (divlji tip) posljedica su taloženja dva pigmenta, smeđeg ksantomatina i svjetlo crvenog pigmenata drosopteridina. Geni w, st i bw kodiraju za ABC protein koji sudjeluje u transportu oba pigmenta. Mutacija oba gena, st i bw, koji kodiraju neispravni protein, uzrokuje bijele oči zbog nemogućnosti transporta pigmenta u pigmentne granule unutar stanica omatidija. Mutacija gena bw rezultira smeđim očima dok mutacija gena st rezultira grimiznim očima. Rezultati broja potomaka nije se poklopio sa očekivanim omjerom potomaka F2 generacije 9:3:3:1. Razlozi nepodudarnosti s omjerom su moguće pogreške u radu prilikom prepoznavanja smeđe i crvene boje očiju, križanje već oplođene mušice umjesto djevičanske zatim ovisnost proizvodnje pigmenta o temperaturi, sljepoća bijelih mušica te kompeticija za hranom. Osim određivanja mutacije mušica, provodili smo križanja s ciljem dobivanja homozigota crvenih i smeđih očiju mušica.
Abstract (english)
The aim of this study was to determine whether the white eye mutation in fruit flies Drosophila melanogaster is due to inheritance sex linked gene or it is autosomal mutation. We inoculate 5 males and 5 virgin females in special containers which we provide with recently prepaired base. After crossing the wild type flies (red eyes) and white flies through several generations we counted offsprings and confirm autosomal mutation as we realise 4 different colours of eye white, brown, red and scarlet. Red colour eyes are result of deposition two pigments xanthommatin (brown) and drosopterins (scarlet) in the pigment granules. W, st and bw genes encode ABC transporter protein that is responsible for transporting both pigments brown and scarlet. Mutation of both genes, st and bw, which encode defective protein causes white eyes due to ineffective transport of pigments in the pigment granules within the cell ommatidia. Null mutants of st have reduced capability to take up tryptophan and the result are scarlet eyes. Similarly, mutants with null allel at bw have reduced capability to take up guanine and the result are brown eyes. After counting the offspring of F2 generation the results weren´t similar to expected ratio 9:3:3:1. The reasons for mismatch with the ratio were possible errors in work such as replacement brown eye and scarlet eye flies, commpetition for food, blindness of white eye flies, using non-virgin flies and dependence on temperature of pigment production. Despite determination of mutation in this work we carried out crossing in which we got homozygotes of brown and scarlet flies.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:798207
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-05-15 09:58:55