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undergraduate thesis
Proizvodnja β-glukanaze pomoću Candide utilis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:306865
Gašpar, Nataša
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gašpar, N. (2012). Proizvodnja β-glukanaze pomoću Candide utilis (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:306865

Gašpar, Nataša. "Proizvodnja β-glukanaze pomoću Candide utilis." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:306865

Gašpar, Nataša. "Proizvodnja β-glukanaze pomoću Candide utilis." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:306865

Gašpar, N. (2012). 'Proizvodnja β-glukanaze pomoću Candide utilis', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:306865

Gašpar N. Proizvodnja β-glukanaze pomoću Candide utilis [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:306865

N. Gašpar, "Proizvodnja β-glukanaze pomoću Candide utilis", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:306865

Metadata
TitleProizvodnja β-glukanaze pomoću Candide utilis
Title (english)Synthesis of β-glucanase by Candida utilis
AuthorNataša Gašpar
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2012-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
β-glukanaze kataliziraju hidrolizu polisaharida građenih od glukoznih jedinica međusobno povezanih β-glikozidnim vezama. β-glukanaze su pretežno ekstracelularni enzimi i izlučuju se u tekući medij pri uzgoju mikroorganizama. Za industrijsku proizvodnju β-glukanaza najviše se upotrebljavaju sojevi bakterije Bacillus subtilus i plijesni Aspergillus niger, a upotrebljavaju se najviše u pivarstvu te u prehrambenoj i fermentacijskoj industriji. U znanstveno-istraživačkim laboratorijima se koriste za protoplastiranje stanica u svrhu izolacije intracelularnih proteina ili prilikom genetskih manipulacija, za ispitivanje svojstava i građe stanične stijenke te izolaciju proteina stanične stijenke. U ovom radu ispitana je mogućnost proizvodnje ekstracelularne β-glukanaze pomoću Candide utilis iz fakultetske zbirke mikroorganizama. Proizvedeni enzim je djelomično pročišćen i koncentriran te mu je aktivnost uspoređena sa aktivnosti komercijalnih preparata glukanaza. Ustanovljeno je da ovaj preparat glukanaze uspješno razgrađuje laminarin, ali slabije djeluje na glukan u intaktnim staničnim stijenkama kvasca.
Abstract (english)
β-glucanase catalyze the hydrolysis of polysaccharides built from glucose units connected by β-glycosidic bonds. β-glucanases are predominantly extracellular enzymes and are excreted into the liquid medium during cultivation of microorganisms. For industrial production of β-glucanase most frequently used strains are Bacillus subtilus and Aspergillus niger. β-glucanases are most frequently used in the brewing and food industry. Furthermore, glucanases are used for research purposes to remove cell wall of microorganisms in order to isolate intracellular proteins or to improve genetic manipulation of cells, for investigating the properties and structure of the cell wall and for isolation of the cell wall proteins. In this paper we investigated the possibility to produce extracellular β-glucanase by Candida utilis from the faculty collection of microorganisms. The produced enzyme was partially purified and concentrated, and its activity was compared with the activity of commercial glucanase preparations. It was determined that glucanase produced by C.albicans successfully degrades laminarin, but has a weaker effect on glucan in the intact yeast cell wall.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:306865
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 07:58:52