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undergraduate thesis
Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:504341
Blasina, Valentino
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Blasina, V. (2012). Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:504341

Blasina, Valentino. "Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:504341

Blasina, Valentino. "Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:504341

Blasina, V. (2012). 'Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:504341

Blasina V. Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:504341

V. Blasina, "Molekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:504341

Metadata
TitleMolekularna karakterizacija neočekivanih genetičkih događaja nastalih tijekom zamjene gena u kvascu
Title (english)The molecular characterization of unexpected genetic events arising during gene replacement in yeast
AuthorValentino Blasina
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-07-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Prilikom transformacije kvasca Saccharomyces cerevisiae linearnom molekulom DNA moguće je usmjeriti integraciju transformirajuće DNA u željeni ciljni lokus ukoliko su krajevi transformirajuće DNA homologni ciljnoj regiji u genomu (ciljanje gena). Ukoliko su krajevi transformirajuće DNA nakon sparivanja s homolognom ciljnom regijom, orijentirani jedan od drugoga, očekivani ishod transformacije je zamjena ciljne regije transformirajućom DNA (rekombinacija krajevi-van). Cilj ovog rada bio je odrediti vrstu i učestalost pojedinih aberantnih genetičkih događaja u transformantima kvasca u određenom eksperimentalnom sustavu u kojem je očekivani ishod zamjena gena HIS3 genom LEU2. Aberantni transformanti prethodno su odabrani na temelju auksonografske analize, a u ovom radu podvrgnuti su molekularnoj karakterizaciji metodom hibridizacije DNA po Southernu. Utvrđeno je da je najčešći aberantni događaj duplikacija ciljnog kromosoma (44.44%).
Abstract (english)
During the transformation of yeast Saccharomyces cerevisiae with a linear DNA molecule it is possible to target the integration of the transforming DNA in the desired target locus if the ends of the transforming DNA are homologous to the target region in the genome (gene targeting). If the ends of the transforming DNA are oriented from one another, then the transformation will result with a gene replacement (ends-out recombination). The aim of this study was to determine the type and frequency of each aberrant genetic event in yeast transformants in a specific experimental system in which the expected genetic event was the replacement of HIS3 gene with LEU2 gene. Based on a previously conducted auxanographic analysis, aberrant yeast transformants were selected and subjected to a molecular characterization by DNA hybridization (Southern blot). The results showed that the most common aberrant genetic event was the duplication of the targeted chromosome (44.44%).
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:504341
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 09:26:58