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undergraduate thesis
Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:025295
Buđa, Renata
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Buđa, R. (2012). Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:025295

Buđa, Renata. "Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:025295

Buđa, Renata. "Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:025295

Buđa, R. (2012). 'Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:025295

Buđa R. Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2025 January 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:025295

R. Buđa, "Konstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:025295

Metadata
TitleKonstrukcija mutanta SLX4 kvasca Saccharomyces cerevisiae
Title (english)Construction of mutant SLX4 yeast Saccharomyces cerevisiae
AuthorRenata Buđa
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2012-07-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Palindromske sekvencije prisutne su u genetičkom materijalu svih živih organizama te su vrlo često uključene u regulaciju fundamentalnih staničnih procesa. Međutim, dulji palindromi mogu u DNA formirati rekombinogene sekundarne strukture koje mogu uzrokovati pucanje kromosoma, genetičke prerasporede i genetičke bolesti kod ljudi. Dosadašnji rezultati sugeriraju da bi za nastanak loma u palindromu u uvjetima in vivo mogao biti odgovoran gen SLX4. Upravo zbog toga je cilj ovoga rada bio provesti inaktivaciju navedenog gena u kvaščevom soju BYUHA-8 koji se otprije koristi za istraživanje rekombinogenosti palindroma. U tu je svrhu najprije lančanom reakcijom polimeraze sintetiziran linearni fragment DNA za inaktivaciju gena SLX4 i tim je fragmentom zatim transformiran kvaščev soj BYUHA-8. Molekularna analiza dobivenih transformanata pokazala je da je u svim transformantima došlo do ilegitimne integracije transformirajuće DNA, a uzrok tome bi mogla biti nedovoljna duljina homologija na krajevima korištenog linearnog fragmenta koja je u ovom radu iznosila 45 parova baza.
Abstract (english)
Palindromic sequences are present in the genetic material of all living organisms and are often involved in the regulation of fundamental cellular processes. However, longer palindromes can form recombinogenic secondary structures that can induce chromosomal breaks, genome rearrangements and cause genetic diseases in humans. Previous results suggest that the SLX4 gene product could be responsible for the introduction of breaks in palindromic sequences in vivo. Therefore, the goal of this paper was to inactivate the SLX4 gene in the yeast strain BYUHA-8, which is used to study palindrome recombinogenicity. To achieve this goal, we synthesized the SLX4 disruption cassette by PCR, and used it to transform yeast strain BYUHA-8. Molecular analysis of the transformants demonstrated that in all cases the disruption cassette integrated at random sites in the genome, which could be due to insufficent length of homology on the ends of the disruption cassette used for transformation.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:025295
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 10:27:28