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undergraduate thesis
Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. urn:nbn:hr:159:697096
Kunštek, Sandra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kunštek, S. (2012). Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:697096

Kunštek, Sandra. "Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:697096

Kunštek, Sandra. "Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2012. https://urn.nsk.hr/urn:nbn:hr:159:697096

Kunštek, S. (2012). 'Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:697096

Kunštek S. Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2012 [cited 2024 July 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:697096

S. Kunštek, "Ispitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2012. Available at: https://urn.nsk.hr/urn:nbn:hr:159:697096

Metadata
TitleIspitivanje povezanosti proteolitičkog procesiranja i načina ugradnje proteina Scw10p u staničnu stijenku kvasca Saccharomyces cerevisiae
Title (english)The effect of proteolytic processing on the incorporation of Scw10p in the Saccharomyces cerevisiae cell wall
AuthorSandra Kunštek
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2012-06-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom je radu ispitivan način vezanja Scw10p proteina u staničnu stijenku kvasca Saccharomyces cerevisiae. U stijenku se proteini mogu vezati nekovalentno (Scw proteini) i kovalentno (Ccw proteini), a kovalentno se vežu ili preko GPI-sidra i β-1,6-glukana ili esterskom vezom između karboksilne skupine glutaminske kiseline i hidroksilne grupe glukoze (Pirproteini koji se izoliraju alkalnom hidrolizom). U ovom je radu dokazano da se protein Scw10p u stijenku može vezati kovalentno i nekovalentno, ovisno o posttranslacijskim modifikacijama u koje su vjerojatno uključene japsinske proteaze i proteaza Kex2p.
Abstract (english)
This research was focused on the effect of proteolytic processing of Scw10p on the incorporation of protein in the cell wall of Saccharomyces cerevisiae. Yeast cell wall contains proteins that are noncovalently (Scw-proteins) or covalently (Ccw-proteins) bound to β-1,3- glucan, the latter either through GPI-anchors and β-1,6-glucan, or by alkali labile ester linkages between γ-carboxyl groups of glutamic acid and hydroxyl groups of glucoses (Pir-proteins, extracted by mild alkali). In this research it is proved that the Scw10p protein bound in cell wall covalently or noncovalently, depending on posttranslational modification probably by yapsin proteases and by Kex2p protease.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:697096
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-16 12:39:35