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undergraduate thesis
Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:495200
Zrnić, Klara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zrnić, K. (2011). Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:495200

Zrnić, Klara. "Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:495200

Zrnić, Klara. "Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:495200

Zrnić, K. (2011). 'Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:495200

Zrnić K. Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:495200

K. Zrnić, "Ispitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:495200

Metadata
TitleIspitivanje osjetljivosti autohtonih izolata bakterija mliječne kiseline na različite antibiotike
Title (english)An investigation of the autochthonous lactic acid bacteria susceptibility to different antibiotics
AuthorKlara Zrnić
MentorJasna Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2011-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bakterije mliječne kiseline (BMK) su važne starter kulture u prehrambenoj industriji, a pojedini predstavnici su definirani i kao probiotici. Povećana primjena antibiotika utjecala je na širenje antibiotičke rezistencije, stoga su istraživanja osjetljivosti potencijalnih probiotičkih sojeva na antibiotike dio općih izbornih kriterija u okviru strogo definiranog probiotičkog koncepta. U ovom radu je provedena karakterizacija pojedinih sojeva autohtone populacije bakterija mliječne kiseline (BMK), izoliranih iz tradicionalno proizvedenog sira, kao probiotičkih starter kultura. Iz svježeg sira odabrano je 19 različitih autohtonih izolata koji su identificirani kao predstavnici BMK, te je ispitana njihova osjetljivost na različite antibiotike metodom difuzije u agaru s filter diskovima. Svi ispitivani sojevi su osjetljivi na ampicilin, bacitracin, kloramfenikol, klindamicin, eritromicin, gentamicin, novobiocin i penicilin G. Kod autohtonih izolata označenih kao BGGO7 i BGG011 nije bila prisutna zona inhibicije rasta nakon primjene vankomicina i streptomicina. U konačnici se može zaključiti da se osjetljivost pojedinog autohtonog izolata na određeni antibiotik razlikuje ovisno o soju mikroorganizma, te je potrebno provesti detaljnije analize.
Abstract (english)
Lactic acid bacteria are important starter cultures in food industry and some representatives are also defined as probiotics. The uncontrolled use of antibiotics had influenced on a spreading of antibiotic resistance, hence the testing of the susceptibility of potential probiotic strains to antibiotics is an important general selective criterion inside the probiotic concept investigations. In this study the characterization of the several autochthonous lactic acid bacteria (LAB) isolates was undertaken in order to select potential probiotic starter cultures isolated from traditionally made cheese. 19 different autochthonous isolates identified as representatives of LAB were selected and their susceptibility to different antibiotics was tested by filter disc assay method. All tested strains were susceptible to ampicillin, bacitracin, chlorampheniol, clindamycin, erytromycin, novobiocin and penicillin G. The zone diameter indicating the inhibition of bacteria growth was absent for the all autochthonous isolated from group BGGO7 and BGG011 when vancomycin and streptomycin were tested. Finally, the susceptibility to the specific antibiotic was variable and depending on the species, hence more detailed analysis would be undertaken.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:495200
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-05-18 13:29:48