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undergraduate thesis
Učinak kanolola iz repičinog ulja na kulture stanica
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:797549
Bagović, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bagović, M. (2016). Učinak kanolola iz repičinog ulja na kulture stanica (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:797549

Bagović, Martina. "Učinak kanolola iz repičinog ulja na kulture stanica." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:797549

Bagović, Martina. "Učinak kanolola iz repičinog ulja na kulture stanica." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:797549

Bagović, M. (2016). 'Učinak kanolola iz repičinog ulja na kulture stanica', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:797549

Bagović M. Učinak kanolola iz repičinog ulja na kulture stanica [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:797549

M. Bagović, "Učinak kanolola iz repičinog ulja na kulture stanica", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:797549

Metadata
TitleUčinak kanolola iz repičinog ulja na kulture stanica
Title (english)The effect of canolol from rapeseed oil on the cell culture
AuthorMartina Bagović
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2016-07-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
4-vinil-2,6-dimetoksifenol ili kanolol je polifenolni spoj izoliran iz nerafiniranog repičinog ulja. Prema dosadašnjim studijama kanolol je pokazao izrazita antioksidacijska svojstva, te je dokazano da ima antimutagenu, antikancerogenu i protuupalnu aktivnost. U ovome radu isptivao se antitumorski utjecaj kanolola in vitro, odnosno u kulturi stanica. Kulture stanica se često koriste za ispitivanje biološke aktivnosti prirodnih spojeva, kao i citotoksičnosti istih. Korištene su dvije humane stanične linije, tumorska (HeLa) i stanična linija izvedena iz zdravih stanica (HEK293T). Obje linije su bile izložene različitim koncentracijama kanolola tijekom 72 sata kako bi se potvrdila njegova antitumorska aktivnost i odredile EC50 vrijednosti spoja. Učinak kanolola na HeLa i HEK293T stanice određen je primjenom kolorimetrijske Neutral Red metode, te vizualiziran bojanjem bojom kristal-ljubičasto. Citotoksičan učinak kanolola zapažen je na obje stanične liniije pri koncentracijama >50µM, proporcionalan je povećanju koncentracije kanolola, a izračunate EC50 vrijednosti iznose 268,431 µM za HEK293T stanice te 333,569 µM za HeLa stanice.
Abstract (english)
4-vinyl-2,6-dimethoxyphenol or canolol is a phenolic compound extracted from crude rapeseed oil. The latest studies show that canolol has strong antioxidative effect, as well as antimutagenic, anticancerogenic and anti-inflammatory activity. The goal of this final thesis was to determine antitumor activity of canolol in vitro, by using cell culture. Cell culture is a frequently used method in the examination of biological active natural compounds, as well as their cytotoxicity. Two human cell lines were used, tumor cell line (HeLa) and normal one conducted from healthy cells (HEK293T). Both of the lines were exposed to different concentrations of canolol in the period of 72 hours in order to prove its antitumor activity and determine its EC50 value. The effect of canolol on HeLa and HEK293T cells was determine by application of colorimetric Neutral Red method, and visualized by colouring with crystal-purple colour. Cytotoxic effect of canolol was noted on both cell lines at concentration >50µM, and it’s proportionate to the canolol dose, EC50 values are 268.431 µM for HEK293T cells and 333.569 µM of HeLa cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:797549
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-04-24 09:30:12