prikaz prve stranice dokumenta Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom
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master's thesis
Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:776409
Bartolić, Tamara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bartolić, T. (2011). Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:776409

Bartolić, Tamara. "Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:776409

Bartolić, Tamara. "Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:776409

Bartolić, T. (2011). 'Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 October 2024, https://urn.nsk.hr/urn:nbn:hr:159:776409

Bartolić T. Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 October 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:776409

T. Bartolić, "Određivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:776409

Metadata
TitleOdređivanje citokina i β-defenzina elisa tehnikom u djece s akutnom respiratornom infekcijom
Title (english)Determination of citokines and β-defensines in children with acute respiratory infection using elisa
AuthorTamara Bartolić
MentorLidija Šver (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2011-02-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Respiratorni sustav neprestano je izložen brojnim štetnim agensima, mikroorganizmima, česticama i alergenima. U uklanjanju i obrani domaćina od navedenih čimbenika sudjeluju komponente nespecifične i specifične imunosti. Nespecifična imunost uključuje brojne mehanizme, a jedan od njih su antimikrobni peptidi koji posjeduju sposobnost inaktivacije različitih mikroorganizama. Oni mogu biti stalno prisutni ili potaknuti na ekspresiju u epitelnim stanicama (posebice u epitelu dišnih puteva). Važna porodica antimikrobnih peptida su β-defenzini. Epitelne stanice izvor su konstitutivno eksprimiranog β-defenzina-1 dok proupalni citokini potiču ekspresiju β-defenzina-2. U ovom istraživanju, odredili smo povišenu koncentraciju β-defenzina-1 i β- defenzina-2 u uzorcima bronhoalveolarnih ispiraka djece s akutnom respiratornom infekcijom uzrokovanom respiratornim sincicijskim virusom, u odnosu na bakterijama uzrokovanu infekciju. Iako nismo našli statističke razlike u koncentracijama proupalnih citokina TNF-α, IL-1β i IL-6 između djece s virusnom i bakterijskom infekcijom, ustvrdili smo pozitivnu korelaciju ekspresije humanog β-defenzina-1 i TNF-α te humanog β-defenzina-2 i IL-1β.
Abstract (english)
The respiratory tract is continuously exposed to numerous noxious agents, microbial organisms and allergens. Components of innate and specific defense mechanisms participate in elimination and host defense of mentioned factors. The innate host defense include many mechanisms, and one of them are antimicrobial peptides. They are widely expressed and rapidly induced at epithelial surfaces (expecially in respiratory system). Important family of antimicrobial peptides are β-defensins. The epithelial cells are a source of constitutively produced β-defensin-1 whereas proinflammatory cytokines induce expression of β-defensin-2. In this study,we determined increased levels of hBD1 and hBD2 in bronchoalveolar lavate samples from children with the acute virus respiratory infection compared to the bacterial respiratory infection group. Although we have not found statistically different TNF-α, IL-1β and IL-6 levels between children with viral or bacterial respiratory infections, there was a positive correlation between hBD1 and TNF-α as well hBD2 and IL-1β levels in BAL.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:776409
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-30 08:25:06