prikaz prve stranice dokumenta Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92
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master's thesis
Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:894158
Dite, Draženka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Dite, D. (2011). Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:894158

Dite, Draženka. "Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:894158

Dite, Draženka. "Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:894158

Dite, D. (2011). 'Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:894158

Dite D. Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:894158

D. Dite, "Funkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:894158

Metadata
TitleFunkcionalna uloga S-layer proteina u probiotičkoj aktivnosti bakterije Lactobacillus helveticus M92
Title (english)Functionality of the S-layer protein from the probiotic strain Lactobacillus helveticus M92
AuthorDraženka Dite
MentorJasna Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2011-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Probiotička bakterija Lactobacillus helveticus M92 sintetizira površinski S-layer protein (SlpA) čija biološka uloga još uvijek nije u potpunosti objašnjena. Stoga je cilj ovoga rada ispitati funkcionalnu ulogu S-layer proteina bakterije L. helveticus M92 u probiotičkom djelovanju L. helveticus M92. Nakon što je prisutnost SlpA proteina utvrđena pomoću SDS-PAGE površinskih proteina i primjenom specifičnih početnica za slpA L. helveticus, istražena je uloga ovog proteina u adheziji L. helveticus M92 na Caco-2 epitelne stanice. Provedena je ekstrakcija SlpA proteina s površine stanica L. helveticus M92 pomoću 5M litijeva klorida, te je uspoređena adhezija bakterijskih stanica L. helveticus M92 na Caco-2 epitelne stanice, prije i nakon uklanjanja S-layer proteina s površine bakterijske stanice. Uklanjanje S-layer proteina s površine stanice L. helveticus M92 utjecao je na smanjenje adhezije ove probiotičke bakterije na Caco-2 epitelne stanice. Također je ispitana koagregacija ovog probiotičkog soja sa Salmonella Typhimurium FP1, istražena uloga S-layer proteina u koagregaciji i kompetitivnoj ekskluziji S. Typhimurium FP1 na Caco-2 epitelne stanice. Iz dobivenih rezultata uočljiva je inhibicija adhezije S. Typhimurium FP1 u prisustvu L. helveticus M92.
Abstract (english)
The probiotic strain Lactobacillus helveticus M92 synthesizes surface S-layer protein (SlpA) whose biological function has not yet been complettly explained. Therefore, the aim of this paper is to investigate functionality of the S-layer protein from the probiotic strain L. helveticus M92 in the probiotic activity of this strain. The presence of SlpA protein was identified by SDS-PAGE of surface proteins and by PCR with a primers specific for slpA of L. helveticus. A role of this protein in adhesion of L. helveticus M92 at Caco-2 epithelial cells was also examined. SlpA protein was extracted from the cell surface of L. helveticus M92 by 5 M lithium chloride. The adhesion of L. helveticus M92 bacterial cells to Caco-2 cell line was compared before and after the removal of S-layer protein from the bacterial surface. The removal of S-layer protein from L. helveticus M92 bacterial surface reduced the adhesion of this probiotic strain to Caco-2 epithelial cells. Coaggregation of this probiotic strain with S. Typhiumurium FP1 was also performed and the role of S-layer protein in coaggregation and competitive exclusion of S. Typhimurium FP1 at Caco-2 epithelial cells was investigated. The inhibition of adhesion of S. Typhimurium FP1 in the presence of L. helveticus M92 was obvious from the results.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:894158
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-30 12:47:35