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undergraduate thesis
Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:909818
Radišić, Veronika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Radišić, V. (2016). Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:909818

Radišić, Veronika. "Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:909818

Radišić, Veronika. "Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:909818

Radišić, V. (2016). 'Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:909818

Radišić V. Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 August 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:909818

V. Radišić, "Utjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:909818

Metadata
TitleUtjecaj glutena na adheziju probiotičkih sojeva na CACO-2 crijevne epitelne stanice
Title (english)The impact of gluten on adhesion of probiotic strains at CACO-2 epithelial intestinal cells
AuthorVeronika Radišić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2016-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Adhezija bakterijskih stanica na epitelne stanice gastrointestinalnog trakta je neophodan uvjet za kolonizaciju i jedan je od najvažnijih izbornih kriterija pri odabiru probiotičkih sojeva. Stoga je cilj ovoga rada bio ispitati utjecaj glutena (γ=1 mg/mL) na in vitro adheziju Lactobacillus sojeva na Caco-2 crijevne epitelne stanice čovjeka. Prema dobivenim rezultatima, primjenjena koncentracija glutena nije utjecala na adheziju probiotičkih sojeva na Caco-2 crijevne epitelne stanice. Svi ispitani Lactobacillussojevi (L. helveticus M92, L. brevis D6 i L. paraplantarum SF9B) su se adhezirali na Caco-2 crijevne epitelne stanice, bez obzira na dodatak glutena. Također, nije ustanovljen utjecaj glutena na preživljavanje ispitanih bakterija mliječne kiseline tijekom 2h inkubacije u fiziološkoj otopini.
Abstract (english)
The adhesion of bacterial cells to epithelian cells of the gastrointestinal tract (GIT) is an indispensable requirement for colonization and one of the most important criteria in the selection of probiotic strains. Therefore, the aim of this study was to examine the impact of gluten (γ=1 mg/mL) on in vitro adhesion of Lactobacillus strains to Caco-2 human epithelial cells. According to the results, applied concentration of gluten did not affect on adhesion of probiotic strains at Caco-2 epithelial intestinal cells. All of the Lactobacillus strains (L. helveticus M92, L. brevis D6 and L. paraplantarum SF9B) have shown the adhesion at Caco-2 epithelial intestinal cells, regardless of the addition of gluten. Also, it has not been established the impact of gluten on the survival of tested lactic acid bacteria during 2h incubation in saline solution.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:909818
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 10:14:32