prikaz prve stranice dokumenta Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae
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master's thesis
Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:575662
Šćulac, Elena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Šćulac, E. (2011). Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:575662

Šćulac, Elena. "Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:575662

Šćulac, Elena. "Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:575662

Šćulac, E. (2011). 'Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:575662

Šćulac E. Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 July 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:575662

E. Šćulac, "Karakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:575662

Metadata
TitleKarakterizacija rekombinantnog proteina Rny1p/Ccw12p eksprimiranog u kvascu Saccharomyces cerevisiae
Title (english)Characterisation of the recombinant protein Rny1p/Ccw12p expressed on the cell wall of yeast Saccharomyces cerevisiae
AuthorElena Šćulac
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2011-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu je katakteriziran rekombinantni protein Rny1p/Ccw12p koji je eksprimiran na staničnoj stijenci kvasca Saccharomyces cerevisiae. Stanična stijenka je organela koja stanici kvasca daje mehaničku i osmotsku stabilnost. Proteini mogu biti vezani na staničnu stijenku kovalentno ili nekovalentno. Kovalentna imobilizacija heterolognih proteina na staničnoj stijenci kvasca ima vrlo važnu ulogu u farmaceutskoj, kemijskoj, prehrambenoj industriji i u industriji detergenata. Protein karakteriziran u ovom radu je rekombinantni protein Rny1p/Ccw12p. Nativni Rny1 protein spada u skupinu T2RNaza u kvascu, ali homolozi ovog proteina pronađeni su i u svim drugim organizmima od proteozoa do životinja. U ovom radu ispitana je lokalizacija dobivenog rekombinantnog proteina, njegova aktivnost, stabilnost i određeni su optimalni pH i temperatura za provođenje enzimske reakcije.
Abstract (english)
In this work the recombinant protein Rny1p/Ccw12p which is expressed on the cell wall of yeast Saccharomyces cerevisiae is characterised. The cell wall is the organelle which provide mechanical and osmotic stability of the yeast cell. Proteins can be bound to the cell wall covalently or noncovalently. Covalent immobilization of heterologous proteins on yeast cell wall plays an important role in the pharmaceutical, chemical, food processing industry and industry of detergents. Native Rny1 protein belongs to the group T2RNase in yeast, homologues of this protein were found in all other organisms. This work examined the localization of the recombinant protein, its activity and stability, determineted optimum pH and temperature for the enzymatic reaction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:575662
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-31 10:43:44