prikaz prve stranice dokumenta Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina
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master's thesis
Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. urn:nbn:hr:159:956909
Glavina, Dalija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Glavina, D. (2011). Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:956909

Glavina, Dalija. "Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:956909

Glavina, Dalija. "Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2011. https://urn.nsk.hr/urn:nbn:hr:159:956909

Glavina, D. (2011). 'Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:956909

Glavina D. Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2011 [cited 2024 June 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:956909

D. Glavina, "Primjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2011. Available at: https://urn.nsk.hr/urn:nbn:hr:159:956909

Metadata
TitlePrimjena CCO stanične linije u istraživanjima citotoksičnosti ionskih tekućina
Title (english)Application of CCO cell line in the study of ionic liquids cytotoxicity
AuthorDalija Glavina
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2011-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Interakcije kemijskih kontaminanata i bioloških sustava najprije se odvijaju na staničnoj razini pri čemu primjena in vitro staničnih kultura može biti vrijedan alat za ranu detekciju kemijskog izlaganja. Posljednjih godina ionske tekućine zaokupljaju pažnju znanstvenika i industrije jer se koriste kao zamjena za hlapiva organska otapala (VOCs). Obzirom na nisku hlapljivost i nezapaljivost, postojane su u okolišu pa je prije šire primjene potrebno odrediti njihovu moguću toksičnost. U ovom radu prikazani su rezultati toksičnih učinaka odabranih ionskih tekućina na proliferaciju stanične linije CCO određeni WST-1 metodom. Na temelju izračunatih EC50 vrijednosti redoslijed toksičnosti odabranih imidazolijevih ionskih tekućina bio je: [C10MIM][Tf2N] > [C7MIM][Tf2N] > [C3- 3MIM][Tf2N] > [C3-2MIM][Tf2N] > [C4MIM][Tf2N] > [C4MIM][PF6] > [C4MIM][BF4]. Toksični učinci ispitanih ionskih tekućina na stanicama CCO bili su mnogo izraženiji od učinaka konvencionalnih organskih otapala. Morfološke promjene inducirane djelovanjem ionskih tekućina na stanice CCO pokazale su promjene u monosloju i strukturi stanica, a zapažena citotoksičnost uglavnom je bila posljedica nekroze.
Abstract (english)
Interactions of chemical contaminants and biological systems at first take place at the cellular level where the application of in vitro cell cultures can be a valuable tool for early detection of chemical exposure. Recently, ionic liquids occupy the attention of scientists and industry for usage as the replacements for volatile organic compounds (VOCs). Considering their low volatility and inflammability they are persistant in the environment. Therefore it is necessary to determine their possible toxicity before their wider application. This paper presents the results of toxic effects of selected ionic liquids on the proliferation of fish cell line CCO determined by the WST-1 method. Based on the calculated EC50 values the order of toxicity of selected imidazolium ionic liquids was: [C10MIM][Tf2N] > [C7MIM][Tf2N] > [C3- 3MIM][Tf2N] > [C3-2MIM][Tf2N] > [C4MIM][Tf2N] > [C4MIM][PF6] > [C4MIM][BF4]. Toxic effects of ionic liquids tested on cells CCO were much more expressed than the effects of conventional organic solvents. Morphological changes induced by the action od ionic liquids on cells CCO have shown changes on the structure of monolayers of cells while the noticed cytotoxicity was largely the result of necrosis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:956909
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-05-31 13:09:41