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undergraduate thesis
Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:274205
Kuhar, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kuhar, M. (2010). Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:274205

Kuhar, Martina. "Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:274205

Kuhar, Martina. "Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:274205

Kuhar, M. (2010). 'Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:274205

Kuhar M. Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2025 January 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:274205

M. Kuhar, "Učinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:274205

Metadata
TitleUčinak 17β-Estradiola na proliferaciju CCO I RTG-2 staničnih linija riba
Title (english)The effect of 17β-Estradiol on proliferation of CCO I RTG-2 fish cell lines
AuthorMartina Kuhar
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2010-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Veliki broj prirodnih, farmaceutskih i sintetskih spojeva s estrogenim učinkom ispušta se u okoliš putem komunalnih i industrijskih otpadnih voda, pa izazivaju promjene rasta, razvoja, ponašanja i reproduktivnih sposobnosti organizama koji žive u vodi. U cilju određivanja i praćenja njihovih učinaka razvijeni su in vitro sustavi koji uključuju i testove citotoksicnosti sa stanicama riba. Stanicne linije CCO (Channel catfish ovary) i RTG-2 (Rainbow trout gonades) dobivene su iz primarne kulture ovarija mladog kanalskog soma, odnosno gonada juvenilne pastrve (Salmo giardneri) i primijenjene su u ispitivanju utjecaja 17β-estradiola na proliferaciju stanica. Na temelju provedenih istraživanja može se zaključiti da dodatak 17β-estradiola smanjuje proliferaciju CCO i RTG-2 staničnih linija proporcionalno koncentraciji. CCO i RTG-2 stanične linije su pogodne za primjenu u ekotoksikološkim ispitivanjima.
Abstract (english)
Large number of natural, pharmaceutical and synthetic compounds with estrogen effect are released into the environment through municipal and industrial wastewater causing changes of growth, development and reproductive capacities for organisms who live in the water. To determine and monitor their effects, scientists developed in vitro systems which include tests of cytotoxicity with fish cells. CCO (Channel catfish ovary) and RTG-2 (Rainbow trout gonades) cell lines were obtained from the primary culture of Channel catfish ovary and Rainbow trout gonades and they were applied to study the effect of 17β-estradiol on cell proliferation. Based on the research it can be concluded that 17β-estradiol reduces proliferatin of CCO and RTG-2 cells showing concentration-dependenty. CCO and RTG-2 cell lines are suitable for use in ecotoxicology.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:274205
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-06-07 09:33:30