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undergraduate thesis
Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. urn:nbn:hr:159:393674
Miletić, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Toxicology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Miletić, M. (2010). Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:393674

Miletić, Marina. "Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:393674

Miletić, Marina. "Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2010. https://urn.nsk.hr/urn:nbn:hr:159:393674

Miletić, M. (2010). 'Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:393674

Miletić M. Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2010 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:393674

M. Miletić, "Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2010. Available at: https://urn.nsk.hr/urn:nbn:hr:159:393674

Metadata
TitlePrimjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika
Title (english)Application of animal cell cultures in toxicity assessment of xenobiotics
AuthorMarina Miletić
MentorIvana Kmetič (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Toxicology)
Zagreb
Defense date and country2010-07-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Kulture životinjskih stanica se koriste u industrijskoj biotehnologiji te u brojnim znanstvenim disciplinama, između ostalog i kao test sustavi u cilju ispitivanja toksičnosti ksenobiotika. U farmaceutskoj industriji njihova primjena vrlo je važna pri proizvodnji visokovrijednih pripravaka poput humanih i veterinarskih cijepiva, hormona, neuroregulatora i sl. Uključivanje in vitro metoda u toksikološka ispitivanja nužno je u cilju smanjenja broja životinja u pokusima. Osim toga, primjena kulture stanica omogućuje istraživanja molekularnih mehanizama toksičnosti, interakcija s receptorima i praćenje specifičnih unutarstaničnih promjena izazvanih djelovanjem toksikanta, dok in vivo studije ne omogućuju takve spoznaje. Većina alternativnih testova toksičnosti još je u postupku razvoja i validacije te se koriste samo kao predtestovi. U ovom radu preliminarno je ispitana mogućnost korištenja CHO-K1 stanične linije kao alternativnog test sustava u ispitivanju citotoksičnosti PCB-77 Trypan Blue metodom. Upotreba kulture CHO-K1 stanica omogućila je vrlo brzo, rutinski i relativno jeftino dobivanje osnovnih podataka o djelovanju odabranog kongenera PCB-a, čime se ovakav test sustav pokazao zadovoljavajućim.
Abstract (english)
Animal culture cells are used in industrial biotechnology and in many scientific disciplines to investigate the toxicity of xenobiotics. In the pharmaceutical industry, their application is very important in producing high-valuable preparations such as human and veterinary vaccines, hormones, neuroregulators etc. Inclusion of in vitro methods in toxicological invastigations is necessary to reduce the number of animals used in experiments. In addition, the use of cell culture allows investigation of molecular mechanisms of toxicity, interaction with receptors and monitoring of specific intracellular changes caused by the activity of toxicant, whereas in vivo studies do not provide such insights. The majority of alternative toxicity tests are still in the process of development and validation and are used only as preliminary tests. In this work it has been investigated the possibility of CHO-K1 cell line usage as an alternative test-system in studying the cytotoxicity of PCB-77 by Trypan Blue method. Utilisation of CHO-K1 culture enabled a very quick, relatively inexpensive and routinely obtaining of basical data on the activities of selected congener of PCBs, and this test-system has been proved as satisfactory.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:393674
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
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Created on2017-06-07 11:59:12