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undergraduate thesis
Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:149500
Berljavac, Sara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Berljavac, S. (2017). Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:149500

Berljavac, Sara. "Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:149500

Berljavac, Sara. "Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:149500

Berljavac, S. (2017). 'Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:149500

Berljavac S. Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 August 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:149500

S. Berljavac, "Djelovanje nesteroidnih protuupalnih spojeva na humane stanične linije", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:149500

Metadata
TitleDjelovanje nesteroidnih protuupalnih spojeva na humane stanične linije
Title (english)The effect of nonsteroidal anti-inflammatory drugs on human cell lines
AuthorSara Berljavac
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nesteroidni protuupalni lijekovi imaju niz farmakoloških učinka, a u posljednje vrijeme sve je više dokaza u prilog njihove primjene kao antikancerogenih lijekova. Oni mogu umanjiti ili zaustaviti proliferaciju stanica oštećenih slobodnim radikalima, koja može voditi do karcinoma, prvenstveno inhibicijom aktivnosti ciklooksigenaze, enzima ključnog u nizu fizioloških i upalnih procesa. Unatoč njihovoj dokazanoj primjeni kao lijekova protiv karcinoma i nadalje se postavljaju pitanja o njihovoj toksičnosti, sigurnosti i učinkovitosti. U ovom radu ispitan je učinak četiri nesteroidna protuupalna lijeka (ketoprofen, karprofen, fluniksin i 4-metilaminoantipirin monohidrat) primjenom in vitro metode i kultura stanica. Preživljenje stanica HEK293T i HepG2 određeno je primjenom kolorimetrijske MTS metode. Na temelju dobivenih rezultata možemo zaključiti da ispitani lijekovi zaista imaju antiproliferativni učinak na tumorsku staničnu liniju, ali djeluju i na staničnu liniju podrijetlom iz zdravog tkiva. Također, zapaženi inhibitorni učinak ovisi o primjenjenoj dozi te o otapalu u kojem je otopljena djelatna tvar.
Abstract (english)
Non-steroidal anti-inflammatory drugs have a number of pharmacological effects and recently there are evidences of their use as anticancer drugs. They can reduce or stop the proliferation of free radical-depleted cells, which could lead to a cancer, primarily by inhibiting cyclooxygenase activity, a key enzyme in a number of physiological and inflammatory processes. Despite their proven use as a anticancer drug, further questions are raised about their toxicity, safety and efficiency. In this work, four nonsteroidal anti-inflammatory drugs (ketoprofen, carprofen, flunixin and 4-methylaminoantipyrine monohydrate) were tested using the in vitro method and cell culture. The survival of HEK293T and HepG2 cells was determined using a colorimetric MTS method. Based on the obtained results we can conclude that the tested drugs indeed have an antiproliferative effect on the tumor cell line, but also have effect on the cell line from a healthy tissue. The observed inhibitory effect depends on the administered dose and the solvent in which the active substance is dissolved.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:149500
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-07-07 12:00:50